TY - JOUR
T1 - Effect of vitamin D deficiency on lipid composition and calcium transport in basolateral membrane vesicles from chick intestine
AU - Alisio, Arturo
AU - Cañas, Fernando
AU - De Bronia, Delia H.
AU - Pereira, Rodolfo
AU - Tolosa De Talamoni, Nori
PY - 1997/6
Y1 - 1997/6
N2 - Vitamin D deficiency affects the lipid composition and Ca2+ uptake of intestinal basolateral membranes from chick intestine. The increased cholesterol content causes an increase in the molar ratio cholesterol/phospholipid. Phospholipid classes remain unchanged, but the percentages of arachidonic acid from the major phospholipid fractions are increased. After 24 hours of oral administration of 2000 IU of cholecalciferol to vitamin D-deficient chicks, the cholesterol values do not change, but the amount of arachidonic acid returns to normal values. Ca2+ uptake driven by ATP is diminished in vesicles from intestinal basolateral membranes of vitamin D-deficient chicks. Cholecalciferol treatment returns these values to the controls which might be due mainly to the increased number of Ca2+ pump units. In conclusion, changes in lipid composition and in Ca2+ pump caused by vitamin D deficiency seems to play a role in the decrease of vesicular Ca2+ transport. A single dose of cholecalciferol restores only partially the lipid-protein changes produced by vitamin D deficiency.
AB - Vitamin D deficiency affects the lipid composition and Ca2+ uptake of intestinal basolateral membranes from chick intestine. The increased cholesterol content causes an increase in the molar ratio cholesterol/phospholipid. Phospholipid classes remain unchanged, but the percentages of arachidonic acid from the major phospholipid fractions are increased. After 24 hours of oral administration of 2000 IU of cholecalciferol to vitamin D-deficient chicks, the cholesterol values do not change, but the amount of arachidonic acid returns to normal values. Ca2+ uptake driven by ATP is diminished in vesicles from intestinal basolateral membranes of vitamin D-deficient chicks. Cholecalciferol treatment returns these values to the controls which might be due mainly to the increased number of Ca2+ pump units. In conclusion, changes in lipid composition and in Ca2+ pump caused by vitamin D deficiency seems to play a role in the decrease of vesicular Ca2+ transport. A single dose of cholecalciferol restores only partially the lipid-protein changes produced by vitamin D deficiency.
KW - Calcium uptake
KW - Chicks
KW - Intestinal basolateral membrane
KW - Lipid composition
KW - Vitamin D
UR - http://www.scopus.com/inward/record.url?scp=0345267157&partnerID=8YFLogxK
U2 - 10.1080/15216549700202741
DO - 10.1080/15216549700202741
M3 - Article
C2 - 9238533
AN - SCOPUS:0345267157
SN - 1039-9712
VL - 42
SP - 339
EP - 347
JO - Biochemistry and Molecular Biology International
JF - Biochemistry and Molecular Biology International
IS - 2
ER -