Effect of vitamin D deficiency on lipid composition and calcium transport in basolateral membrane vesicles from chick intestine

Arturo Alisio, Fernando Cañas, Delia H. De Bronia, Rodolfo Pereira, Nori Tolosa De Talamoni

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Vitamin D deficiency affects the lipid composition and Ca2+ uptake of intestinal basolateral membranes from chick intestine. The increased cholesterol content causes an increase in the molar ratio cholesterol/phospholipid. Phospholipid classes remain unchanged, but the percentages of arachidonic acid from the major phospholipid fractions are increased. After 24 hours of oral administration of 2000 IU of cholecalciferol to vitamin D-deficient chicks, the cholesterol values do not change, but the amount of arachidonic acid returns to normal values. Ca2+ uptake driven by ATP is diminished in vesicles from intestinal basolateral membranes of vitamin D-deficient chicks. Cholecalciferol treatment returns these values to the controls which might be due mainly to the increased number of Ca2+ pump units. In conclusion, changes in lipid composition and in Ca2+ pump caused by vitamin D deficiency seems to play a role in the decrease of vesicular Ca2+ transport. A single dose of cholecalciferol restores only partially the lipid-protein changes produced by vitamin D deficiency.

Original languageEnglish
Pages (from-to)339-347
Number of pages9
JournalBiochemistry and Molecular Biology International
Volume42
Issue number2
DOIs
StatePublished - Jun 1997

Keywords

  • Calcium uptake
  • Chicks
  • Intestinal basolateral membrane
  • Lipid composition
  • Vitamin D

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