TY - JOUR
T1 - Effect of time and dose of recombinant follicle stimulating hormone agonist on the superovulatory response of sheep
AU - Rutigliano, Heloisa M.
AU - Adams, Betty M.
AU - Jablonka-Shariff, Albina
AU - Boime, Irving
AU - Adams, Thomas E.
N1 - Funding Information:
The authors thank Dr. Carla Rodriguez, Dr. Seth Wegner, Dr. Benjamin Renquist, and Laura Dao for technical assistance. This project was supported by the National Research Initiative Grant no. 2005-35203-16274 from the USDA Cooperative State Research, Education and Extension Service.
PY - 2014/8
Y1 - 2014/8
N2 - The objective of this study was to determine the superovulatory potential of a single-chain analog of human FSH (Fcα) when administered to ewes either 3days before, or coincident with, simulated luteolysis (pessary removal [PR]). A total of 40 animals were randomly assigned to receive Fcα at doses of 0.62, 1.25, or 2.5IU/kg of body weight (bwt) 3days before PR or 0.31, 0.62, 1.25, or 2.5IU/kg of bwt at PR. Control ewes received protein without FSH activity. Blood samples were collected during the periovulatory period and ovarian tissue was collected 11days after PR. Ovulation rate did not differ from the control group in ewes receiving the smallest doses of Fcα (0.31 and 0.62IU/kg). However, a significant superovulatory response was noted in sheep receiving Fcα at doses of 1.25 and 2.5IU/kg and this response was comparable in animals receiving the largest dose levels of Fcα at, or 3days before, PR. The interval between PR and the LH surge was significantly extended and the LH surges were less synchronous in animals receiving Fcα at PR when compared with animals receiving the potent FSH agonist 3days before PR. Taken together, these data indicate that the human single-chain gonadotropin with FSH activity promotes superovulation in ewe lambs in the breeding season. A single injection of the recombinant gonadotropin 3days before luteolysis synchronizes the LH surge. The use of the single-chain analog of FSH in assisted reproduction for domestic animals is likely to be of practical significance as an alternative to conventional gonadotropins in superovulation protocols in livestock species.
AB - The objective of this study was to determine the superovulatory potential of a single-chain analog of human FSH (Fcα) when administered to ewes either 3days before, or coincident with, simulated luteolysis (pessary removal [PR]). A total of 40 animals were randomly assigned to receive Fcα at doses of 0.62, 1.25, or 2.5IU/kg of body weight (bwt) 3days before PR or 0.31, 0.62, 1.25, or 2.5IU/kg of bwt at PR. Control ewes received protein without FSH activity. Blood samples were collected during the periovulatory period and ovarian tissue was collected 11days after PR. Ovulation rate did not differ from the control group in ewes receiving the smallest doses of Fcα (0.31 and 0.62IU/kg). However, a significant superovulatory response was noted in sheep receiving Fcα at doses of 1.25 and 2.5IU/kg and this response was comparable in animals receiving the largest dose levels of Fcα at, or 3days before, PR. The interval between PR and the LH surge was significantly extended and the LH surges were less synchronous in animals receiving Fcα at PR when compared with animals receiving the potent FSH agonist 3days before PR. Taken together, these data indicate that the human single-chain gonadotropin with FSH activity promotes superovulation in ewe lambs in the breeding season. A single injection of the recombinant gonadotropin 3days before luteolysis synchronizes the LH surge. The use of the single-chain analog of FSH in assisted reproduction for domestic animals is likely to be of practical significance as an alternative to conventional gonadotropins in superovulation protocols in livestock species.
KW - Assisted reproductive technologies
KW - Gonadotropin
KW - Multiple ovulation
KW - Ovine
KW - Single-chain gonadotropin
UR - http://www.scopus.com/inward/record.url?scp=84903730380&partnerID=8YFLogxK
U2 - 10.1016/j.theriogenology.2014.05.010
DO - 10.1016/j.theriogenology.2014.05.010
M3 - Article
C2 - 24938801
AN - SCOPUS:84903730380
SN - 0093-691X
VL - 82
SP - 455
EP - 460
JO - Theriogenology
JF - Theriogenology
IS - 3
ER -