Effect of surface modification of silicone on Staphylococcus epidermidis adhesion and colonization

Haiying Tang, Ting Cao, Anfeng Wang, Xuemei Liang, Steven O. Salley, James P. McAllister, K. Y.Simon Ng

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Cerebrospinal fluid (CSF) shunts for the treatment of hydrocephalus are generally made of silicone rubber. The growth of bacterial colonies on the silicone surface leads to frequent CSF shunt complications. A systematic study of the effect of the surface modification of silicone on Staphylococcus epidermidis adhesion and colonization was performed for different incubation times by means of colony counting and scanning electron microscopy (SEM). Silicone was modified with different biopolymers and silanes, including heparin, hyaluronan, octadecyltrichlorosilane (OTS), and fluoroalkylsilane (FAS) to provide a stable and biocompatible surface with different surface functional groups and degrees of hydrophobicity. The modified silicone surfaces were studied by using contact angle measurements, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). After 4 and 8 h of incubation, the FAS- and OTS-coated silicone and the hyaluronan coated OTS/silicone surfaces showed significantly reduced bacterial adhesion and colonization compared to blank silicone by both quantification methods. However, the heparin coated OTS/silicone showed significantly increased bacterial adhesion. These results indicate that the nature of the surface functional group and surface roughness determine the extent of bacterial adhesion and colonization. However, the degree of hydrophobicity of the surface did not appear to play a determining role in bacterial adhesion and colonization.

Original languageEnglish
Pages (from-to)885-894
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume80
Issue number4
DOIs
StatePublished - Mar 15 2007
Externally publishedYes

Keywords

  • Bacterial adhesion
  • Colonization
  • Modified silicone
  • Staphylococcus epidermidis

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