Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate

Mingjie Jin; William Bothfeld; Samantha Austin; Trey K. Sato; Alex La Reau; Haibo Li; Marcus Foston; Christa Gunawan; Richard D. LeDuc; John F. Quensen; Mick Mcgee; Nirmal Uppugundla; Alan Higbee; Ruwan Ranatunga; Charles W. Donald; Gwen Bone; Arthur J. Ragauskas; James M. Tiedje; Daniel R. Noguera; Bruce E. Dale; Yaoping Zhang; Venkatesh Balan

doi:10.1016/j.biortech.2013.08.018

Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate

Mingjie Jin
, William Bothfeld
, Samantha Austin
, Trey K. Sato
, Alex La Reau
, Haibo Li
, Marcus Foston
, Christa Gunawan
, Richard D. LeDuc
, John F. Quensen
, Mick Mcgee
, Nirmal Uppugundla
, Alan Higbee
, Ruwan Ranatunga
, Charles W. Donald
, Gwen Bone
, Arthur J. Ragauskas
, James M. Tiedje
, Daniel R. Noguera
, Bruce E. Dale

Yaoping Zhang, Venkatesh Balan

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

To minimize the change of lignocellulosic hydrolysate composition during storage, the effects of storage conditions (temperature, pH and time) on the composition and fermentability of hydrolysate prepared from AFEX™ (Ammonia Fiber Expansion - a trademark of MBI, Lansing, MI) pretreated corn stover were investigated. Precipitates formed during hydrolysate storage increased with increasing storage pH and time. The precipitate amount was the least when hydrolysate was stored at 4°C and pH 4.8, accounting for only 0.02% of the total hydrolysate weight after 3-month storage. No significant changes of NMR (Nuclear Magnetic Resonance) spectra and concentrations of sugars, minerals and heavy metals were observed after storage under this condition. When pH was adjusted higher before fermentation, precipitates also formed, consisting of mostly struvite (MgNH₄PO₄·6H₂O) and brushite (CaHPO₄·2H₂O). Escherichia coli and Saccharomyces cerevisiae fermentation studies and yeast cell growth assays showed no significant difference in fermentability between fresh hydrolysate and stored hydrolysate.

Original language	English
Pages (from-to)	212-220
Number of pages	9
Journal	Bioresource Technology
Volume	147
DOIs	https://doi.org/10.1016/j.biortech.2013.08.018
State	Published - Nov 2013

Keywords

AFEX
Hydrolysate characterization
Hydrolysate fermentation
Hydrolysate precipitate
Hydrolysate storage

Access to Document

10.1016/j.biortech.2013.08.018

Cite this

Jin, M., Bothfeld, W., Austin, S., Sato, T. K., La Reau, A., Li, H., Foston, M., Gunawan, C., LeDuc, R. D., Quensen, J. F., Mcgee, M., Uppugundla, N., Higbee, A., Ranatunga, R., Donald, C. W., Bone, G., Ragauskas, A. J., Tiedje, J. M., Noguera, D. R., ... Balan, V. (2013). Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate. Bioresource Technology, 147, 212-220. https://doi.org/10.1016/j.biortech.2013.08.018

@article{2df5fb2c8e114ababbd85e70831e43ca,

title = "Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate",

abstract = "To minimize the change of lignocellulosic hydrolysate composition during storage, the effects of storage conditions (temperature, pH and time) on the composition and fermentability of hydrolysate prepared from AFEX{\texttrademark} (Ammonia Fiber Expansion - a trademark of MBI, Lansing, MI) pretreated corn stover were investigated. Precipitates formed during hydrolysate storage increased with increasing storage pH and time. The precipitate amount was the least when hydrolysate was stored at 4°C and pH 4.8, accounting for only 0.02\% of the total hydrolysate weight after 3-month storage. No significant changes of NMR (Nuclear Magnetic Resonance) spectra and concentrations of sugars, minerals and heavy metals were observed after storage under this condition. When pH was adjusted higher before fermentation, precipitates also formed, consisting of mostly struvite (MgNH4PO4·6H2O) and brushite (CaHPO4·2H2O). Escherichia coli and Saccharomyces cerevisiae fermentation studies and yeast cell growth assays showed no significant difference in fermentability between fresh hydrolysate and stored hydrolysate.",

keywords = "AFEX, Hydrolysate characterization, Hydrolysate fermentation, Hydrolysate precipitate, Hydrolysate storage",

author = "Mingjie Jin and William Bothfeld and Samantha Austin and Sato, \{Trey K.\} and \{La Reau\}, Alex and Haibo Li and Marcus Foston and Christa Gunawan and LeDuc, \{Richard D.\} and Quensen, \{John F.\} and Mick Mcgee and Nirmal Uppugundla and Alan Higbee and Ruwan Ranatunga and Donald, \{Charles W.\} and Gwen Bone and Ragauskas, \{Arthur J.\} and Tiedje, \{James M.\} and Noguera, \{Daniel R.\} and Dale, \{Bruce E.\} and Yaoping Zhang and Venkatesh Balan",

year = "2013",

month = nov,

doi = "10.1016/j.biortech.2013.08.018",

language = "English",

volume = "147",

pages = "212--220",

journal = "Bioresource Technology",

issn = "0960-8524",

}

Jin, M, Bothfeld, W, Austin, S, Sato, TK, La Reau, A, Li, H, Foston, M, Gunawan, C, LeDuc, RD, Quensen, JF, Mcgee, M, Uppugundla, N, Higbee, A, Ranatunga, R, Donald, CW, Bone, G, Ragauskas, AJ, Tiedje, JM, Noguera, DR, Dale, BE, Zhang, Y & Balan, V 2013, 'Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate', Bioresource Technology, vol. 147, pp. 212-220. https://doi.org/10.1016/j.biortech.2013.08.018

TY - JOUR

T1 - Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate

AU - Jin, Mingjie

AU - Bothfeld, William

AU - Austin, Samantha

AU - Sato, Trey K.

AU - La Reau, Alex

AU - Li, Haibo

AU - Foston, Marcus

AU - Gunawan, Christa

AU - LeDuc, Richard D.

AU - Quensen, John F.

AU - Mcgee, Mick

AU - Uppugundla, Nirmal

AU - Higbee, Alan

AU - Ranatunga, Ruwan

AU - Donald, Charles W.

AU - Bone, Gwen

AU - Ragauskas, Arthur J.

AU - Tiedje, James M.

AU - Noguera, Daniel R.

AU - Dale, Bruce E.

AU - Zhang, Yaoping

AU - Balan, Venkatesh

PY - 2013/11

Y1 - 2013/11

N2 - To minimize the change of lignocellulosic hydrolysate composition during storage, the effects of storage conditions (temperature, pH and time) on the composition and fermentability of hydrolysate prepared from AFEX™ (Ammonia Fiber Expansion - a trademark of MBI, Lansing, MI) pretreated corn stover were investigated. Precipitates formed during hydrolysate storage increased with increasing storage pH and time. The precipitate amount was the least when hydrolysate was stored at 4°C and pH 4.8, accounting for only 0.02% of the total hydrolysate weight after 3-month storage. No significant changes of NMR (Nuclear Magnetic Resonance) spectra and concentrations of sugars, minerals and heavy metals were observed after storage under this condition. When pH was adjusted higher before fermentation, precipitates also formed, consisting of mostly struvite (MgNH4PO4·6H2O) and brushite (CaHPO4·2H2O). Escherichia coli and Saccharomyces cerevisiae fermentation studies and yeast cell growth assays showed no significant difference in fermentability between fresh hydrolysate and stored hydrolysate.

AB - To minimize the change of lignocellulosic hydrolysate composition during storage, the effects of storage conditions (temperature, pH and time) on the composition and fermentability of hydrolysate prepared from AFEX™ (Ammonia Fiber Expansion - a trademark of MBI, Lansing, MI) pretreated corn stover were investigated. Precipitates formed during hydrolysate storage increased with increasing storage pH and time. The precipitate amount was the least when hydrolysate was stored at 4°C and pH 4.8, accounting for only 0.02% of the total hydrolysate weight after 3-month storage. No significant changes of NMR (Nuclear Magnetic Resonance) spectra and concentrations of sugars, minerals and heavy metals were observed after storage under this condition. When pH was adjusted higher before fermentation, precipitates also formed, consisting of mostly struvite (MgNH4PO4·6H2O) and brushite (CaHPO4·2H2O). Escherichia coli and Saccharomyces cerevisiae fermentation studies and yeast cell growth assays showed no significant difference in fermentability between fresh hydrolysate and stored hydrolysate.

KW - AFEX

KW - Hydrolysate characterization

KW - Hydrolysate fermentation

KW - Hydrolysate precipitate

KW - Hydrolysate storage

UR - https://www.scopus.com/pages/publications/84883328960

U2 - 10.1016/j.biortech.2013.08.018

DO - 10.1016/j.biortech.2013.08.018

M3 - Article

C2 - 23999256

AN - SCOPUS:84883328960

SN - 0960-8524

VL - 147

SP - 212

EP - 220

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this