Effect of pre- and postmortem variables on specific mRNA levels in human brain

William J. Burke, Karen L. O'Malley, Hyung D. Chung, Steve K. Harmon, J. Philip Miller, Leonard Berg

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

The method of polymerase chain reaction was used to investigate the pre- and postmortem factors which affect the stability of specific mRNAs in the C1 region of human autopsy brain. Eight premortem and 4 postmortem factors were correlated to levels of phenylethanolamine N-methyltransferase (PNMT), three splice forms of amyloid precursor protein (APP) and actin mRNAs in 10 control brains using Pearson's correlation coefficient. Significant negative correlations were found between hypoxia and PNMT mRNA, and between postmortem and storage intervals and APP751 and β-actin mRNAs. A positive correlation was found between death-refrigeration interval and total APP and APP695 mRNAs. There was also a positive correlation between seizure activity and APP770 mRNA. The results indicate that a variety of pre- and postmortem factors can affect mRNA levels. The possible effect of pre- and postmortem factors on specific mRNA levels should be investigated prior to comparing mRNA levels in different disease states.

Original languageEnglish
Pages (from-to)37-41
Number of pages5
JournalMolecular Brain Research
Volume11
Issue number1
DOIs
StatePublished - Aug 1991

Keywords

  • Human brain
  • Postmortem variable
  • mRNA level

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