Effect of Panax notoginseng saponin on cytochrome P450 mRNA expression in H9c2 cell

Aim: To study the effect of Panax notoginseng saponin on cytochrome P450 mRNA expression in H9c2 cell. Methods: H9c2 cells were treated with different concentrations of PNS for 48h. Cell viability was examined by MTS assay kit cell menbrane injury was examined by detecting the release rate of lactate dehydrogenase (LDH). The expression of cytochrome P450 mRNA was detected by Real Time PCR. Results: H9c2 cell viability significantly reduced following exposure to dictamnine 0.3125-20 g·L^-1 for 48h in a concentration-dependent manner and IC₅₀ value was (5.28 ±0.08) g·L^-1. The LDH release rate of H9c2 cells significantly increased after exposure to dictamnine 0.3125-20 g·L^-1 for 48h (P <0.01). On the mRNA level, compared with the control group, PNS induced the mRNA level of CYP2J3, CYP4A1, CYP4A2, CYP4F1 and CYP4F5 (P <0.05 or P <0.01). PNS had inhibitory activity of CYP2C11, CYP4A3 and CYP4F4 (P <0.05 or P <0.01), while had inductive effects on mRNA of CYP4A3 and CYP4F4 (P <0.01) at 5 g·L^-1. Conclusion: These results indicate that PNS at higher concentrations has potentioal hepatotoxicity. PNS had different effects on the mRNA level of CYP in different concentrations. It might protect heart and blood vessel. Doses of medication should be more careful when PNS is used in clinic.