Effect of light scattering on optical-resolution photoacoustic microscopy

  • Yan Liu
  • , Chi Zhang
  • , Song Hu
  • , Yuta Suzuki
  • , Zhun Xu
  • , Silvina Ferradal
  • , Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

The penetration depth of ballistic optical imaging technologies is limited by light scattering. To study the effect of scattering on optical-resolution photoacoustic microscopy (OR-PAM), we divided the signals in OR-PAM into two classes: one is from the target volume defined by the optical resolution cell (Class I); the other is from the rest of the acoustic resolution cell (Class II). We developed a way to simulate the point spread function (PSF) of our OR-PAM system considering both optical illumination and acoustic detection, then used the PSF to calculate the contributions of each class of signal to the total signal at different focal depths. Our simulation results showed that: 1) The Class II signal decays much more slowly than the Class I signal; 2) The full width at half maximum (FWHM) of the PSF for the focal depth of 0.9 transport mean free path (TMFP) is not broadened much (∼10%) compared with that for a clear medium; 3) Image contrast is degraded with increasing depth when there is a uniform absorption background.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2012
PublisherSPIE
ISBN (Print)9780819488664
DOIs
StatePublished - Feb 9 2012
EventPhotons Plus Ultrasound: Imaging and Sensing 2012 - San Francisco, CA, United States
Duration: Jan 22 2012Jan 24 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8223
ISSN (Print)1605-7422

Conference

ConferencePhotons Plus Ultrasound: Imaging and Sensing 2012
Country/TerritoryUnited States
CitySan Francisco, CA
Period01/22/1201/24/12

Keywords

  • Light scattering
  • Optical-resolution photoacoustic microscopy
  • Penetration depth

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