Effect of Insulin on Amino Acid Uptake and Protein Turnover in Skeletal Muscle From Septic Rats: Evidence for Insulin Resistance of Protein Breakdown

Per Olof Hasselgren, Brad W. Warner, J. Howard James, Hiroo Takehara, Josef E. Fischer

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

• We investigated the effect of different concentrations of insulin (0, 10, 1×102, 1×103, 1×104, and 1×105 mU/L [0, 70, 7×102, 7×103, 7×104, and 7×105 pmol/L]) on amino acid (α-aminoisobutyric acid) uptake and protein synthesis and breakdown in incubated extensor digitorum longus (EDL) and soleus muscles of rats. We studied three groups: (1) untreated, fed rats; (2) sham-operated rats; and (3) septic rats. Sepsis was induced by cecal ligation and puncture. The α-aminoisobutyric acid uptake was increased by insulin in all three groups. Protein synthesis was maximally stimulated by 30% to 40% by 1×102 mU/L (7×102 pmol/L) of insulin in all three groups. Protein degradation in soleus muscle was not affected by insulin. In EDL muscles from untreated and sham-operated rats, protein breakdown was reduced by 15% to 20% by 1 × 102 mU/L (7×102 pmol/L) of insulin. In contrast, protein breakdown was not inhibited by insulin in septic EDL muscle until the concentration of the hormone was increased to 1 ×104 mU/L (7×104 pmol/L), at which concentration the hormonal effect was less than half that in nonseptic muscle. The results suggest a postreceptor insulin resistance of protein breakdown in septic muscle, while the response to the hormone of amino acid transport and protein synthesis was not altered in sepsis.

Original languageEnglish
Pages (from-to)228-233
Number of pages6
JournalArchives of Surgery
Volume122
Issue number2
DOIs
StatePublished - Feb 1987
Externally publishedYes

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