Effect of high MiR-146a expression on the inflammatory reaction in BV2 cells

Objective: To explore the effect of MiR-146a regulator function on the inflammatory response in neuroglia cell (microglia). Methods: BV2 cells were transfected by MiR-146a mimics, and then stimulated by lipopolysaccharide (LPS). MiR-146a expression was measured by real-time polymerase chain reaction (real-time PCR). Interleukin (IL)-6 and tumor necrosis factor a (TNFα) were measured by enzyme-linked immunosorbent assay (ELISA). Furthermore, IL-1 receptor-associated kinase 1 (IRAKI) and TNF receptor-associated factor 6 (TRAF6) were detected by PCR and Western blotting. Results: Compared to the normal control group, MiR-146a expression was significantly elevated by transfection with MiR-146a mimics (t = 5.846, P= 0.0021). The expression levels of IRAKI, TRAF6, TNFa, and IL-6 significantly increased in the LPS-stimulated BV2 cells compared to the non-stimulated BV2. The enhancement of MiR-146a resulted in significantly decreased IL-6 (t = 5. 200, P = 0. 0003) and TNFα (t = 9. 812, P < 0. 0001) secretion. The mRNA (t = 5. 353, P = 0. 0007) and protein (J = 6. 980, P = 0. 0009) levels of TRAF6, but not IRAKI, also significantly decreased. Conclusion: MiR-146a may negatively suppress the inflammatory response of BV2 cells by regulating the expression of IRAF6 molecules in the TLR4 signaling pathway.