Effect of FK506 on peripheral nerve regeneration through long grafts in inbred swine

John N. Jensen, Michael J. Brenner, Thomas H. Tung, Daniel A. Hunter, Susan E. Mackinnon

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Numerous small-animal studies have demonstrated that FK506 enhances nerve regeneration and accelerates functional recovery after nerve injury. However, no experimental study has corroborated these neuroregenerative effects in larger animals. This study investigated the effects of FK506 on nerve regeneration in inbred miniature swine. Eight animals received 8-cm ulnar nerve autografts and allografts. Treated animals received 0.1 to 0.4 mg/kg FK506 injections twice weekly to maintain immunosuppressive serum FK506 levels. At 24 weeks posttransplant, nerve grafts were harvested for histomorphometric analysis. Mixed lymphocyte cultures demonstrated alloreactivity in 1 treated animal and all untreated animals. In autografts, mean fiber count, nerve density, and percent neural tissue were doubled with FK506 therapy. In allografts, significant neuroregeneration was observed in animals treated with FK506, whereas untreated animals had no regeneration. Treatment with K.P. resulted in a trend toward enhanced axonal regeneration through nerve autografts and allografts in a large-animal model with defined histocompatibility barriers.

Original languageEnglish
Pages (from-to)420-427
Number of pages8
JournalAnnals of Plastic Surgery
Volume54
Issue number4
DOIs
StatePublished - Apr 1 2005

Keywords

  • Allograft
  • FK506
  • Immunosuppression
  • Nerve regeneration
  • Tacrolimus

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