TY - JOUR
T1 - Edman sequencing as tool for characterization of synthetic peptides
AU - Grant, Gregory A.
AU - Crankshaw, Mark W.
AU - Gorka, John
PY - 1997
Y1 - 1997
N2 - Sequence analysis of synthetic peptides using Edman chemistry can be very useful for the elucidation of certain types of synthetic problems, such as residue deletions and the presence of common stable derivatives, and for following the progress of the synthesis itself. However, it can also be a relatively poor technique for assessing quantitative aspects and the type and degree of adduct formation that arise from the synthetic chemistry. For these latter considerations, techniques such as mass spectrometry can often give more precise and informative data about the integrity of a synthetic peptide. Thus, sequence analysis is best applied judiciously and then used in combination with other methods. Furthermore, proper interpretation of the results of sequence analysis of synthetic peptides relies on a thorough knowledge of the sequencing process.
AB - Sequence analysis of synthetic peptides using Edman chemistry can be very useful for the elucidation of certain types of synthetic problems, such as residue deletions and the presence of common stable derivatives, and for following the progress of the synthesis itself. However, it can also be a relatively poor technique for assessing quantitative aspects and the type and degree of adduct formation that arise from the synthetic chemistry. For these latter considerations, techniques such as mass spectrometry can often give more precise and informative data about the integrity of a synthetic peptide. Thus, sequence analysis is best applied judiciously and then used in combination with other methods. Furthermore, proper interpretation of the results of sequence analysis of synthetic peptides relies on a thorough knowledge of the sequencing process.
UR - http://www.scopus.com/inward/record.url?scp=0030726339&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(97)89056-8
DO - 10.1016/S0076-6879(97)89056-8
M3 - Article
C2 - 9353730
AN - SCOPUS:0030726339
SN - 0076-6879
VL - 289
SP - 395
EP - 419
JO - Methods in enzymology
JF - Methods in enzymology
ER -