Economic 3D-printing approach for transplantation of human stem cell-derived β-like cells

Jiwon Song, Jeffrey R. Millman

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Transplantation of human pluripotent stem cells (hPSC) differentiated into insulin-producing β cells is a regenerative medicine approach being investigated for diabetes cell replacement therapy. This report presents a multifaceted transplantation strategy that combines differentiation into stem cell-derived β (SC-β) cells with 3D printing. By modulating the parameters of a low-cost 3D printer, we created a macroporous device composed of polylactic acid (PLA) that houses SC-β cell clusters within a degradable fibrin gel. Using finite element modeling of cellular oxygen diffusion-consumption and an in vitro culture system that allows for culture of devices at physiological oxygen levels, we identified cluster sizes that avoid severe hypoxia within 3D-printed devices and developed a microwell-based technique for resizing clusters within this range. Upon transplantation into mice, SC-β cell-embedded 3D-printed devices function for 12 weeks, are retrievable, and maintain structural integrity. Here, we demonstrate a novel 3D-printing approach that advances the use of differentiated hPSC for regenerative medicine applications and serves as a platform for future transplantation strategies.

Original languageEnglish
Article number015002
JournalBiofabrication
Volume9
Issue number1
DOIs
StatePublished - Mar 2017

Keywords

  • 3D printing
  • diabetes
  • fibrin
  • pancreatic
  • scaffold
  • stem cells
  • transplantation

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