EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis

Zhenlin Yang, Shuiming Qian, Ray N. Scheid, Li Lu, Xiangsong Chen, Rui Liu, Xuan Du, Xinchen Lv, Melissa D. Boersma, Mark Scalf, Lloyd M. Smith, John M. Denu, Jiamu Du, Xuehua Zhong

Research output: Contribution to journalLetterpeer-review

90 Scopus citations

Abstract

The ability of cells to perceive and translate versatile cues into differential chromatin and transcriptional states is critical for many biological processes 1–5 . In plants, timely transition to a flowering state is crucial for successful reproduction 6–9 . EARLY BOLTING IN SHORT DAY (EBS) is a negative transcriptional regulator that prevents premature flowering in Arabidopsis thaliana 10,11 . We found that EBS contains bivalent bromo-adjacent homology (BAH)–plant homeodomain (PHD) reader modules that bind H3K27me3 and H3K4me3, respectively. We observed co-enrichment of a subset of EBS-associated genes with H3K4me3, H3K27me3, and Polycomb repressor complex 2 (PRC2). Notably, EBS adopted an autoinhibition mode to mediate its switch in binding preference between H3K27me3 and H3K4me3. This binding balance was critical because disruption of either EBS–H3K27me3 or EBS–H3K4me3 interaction induced early floral transition. Our results identify a bivalent chromatin reader capable of recognizing two antagonistic histone marks, and we propose a distinct mechanism of interaction between active and repressive chromatin states.

Original languageEnglish
Pages (from-to)1247-1253
Number of pages7
JournalNature Genetics
Volume50
Issue number9
DOIs
StatePublished - Sep 1 2018

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