TY - JOUR
T1 - Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis
AU - Xu, Wei
AU - Luthra, Priya
AU - Wu, Chao
AU - Batra, Jyoti
AU - Leung, Daisy W.
AU - Basler, Christopher F.
AU - Amarasinghe, Gaya K.
N1 - Publisher Copyright:
© The Author(s) 2017.
PY - 2017/6/8
Y1 - 2017/6/8
N2 - Ebola virus (EBOV) is an enveloped negative-sense RNA virus that causes sporadic outbreaks with high case fatality rates. Ebola viral protein 30 (eVP30) plays a critical role in EBOV transcription initiation at the nucleoprotein (eNP) gene, with additional roles in the replication cycle such as viral assembly. However, the mechanistic basis for how eVP30 functions during the virus replication cycle is currently unclear. Here we define a key interaction between eVP30 and a peptide derived from eNP that is important to facilitate interactions leading to the recognition of the RNA template. We present crystal structures of the eVP30 C-terminus in complex with this eNP peptide. Functional analyses of the eVP30-eNP interface identify residues that are critical for viral RNA synthesis. Altogether, these results support a model where the eVP30-eNP interaction plays a critical role in transcription initiation and provides a novel target for the development of antiviral therapy.
AB - Ebola virus (EBOV) is an enveloped negative-sense RNA virus that causes sporadic outbreaks with high case fatality rates. Ebola viral protein 30 (eVP30) plays a critical role in EBOV transcription initiation at the nucleoprotein (eNP) gene, with additional roles in the replication cycle such as viral assembly. However, the mechanistic basis for how eVP30 functions during the virus replication cycle is currently unclear. Here we define a key interaction between eVP30 and a peptide derived from eNP that is important to facilitate interactions leading to the recognition of the RNA template. We present crystal structures of the eVP30 C-terminus in complex with this eNP peptide. Functional analyses of the eVP30-eNP interface identify residues that are critical for viral RNA synthesis. Altogether, these results support a model where the eVP30-eNP interaction plays a critical role in transcription initiation and provides a novel target for the development of antiviral therapy.
UR - http://www.scopus.com/inward/record.url?scp=85020687433&partnerID=8YFLogxK
U2 - 10.1038/ncomms15576
DO - 10.1038/ncomms15576
M3 - Article
C2 - 28593988
AN - SCOPUS:85020687433
SN - 2041-1723
VL - 8
JO - Nature communications
JF - Nature communications
M1 - 15576
ER -