Early detection of myocardial reperfusion by assay of plasma MM-creatine kinase isoforms in dogs

S. R. Devries, B. E. Sobel, D. R. Abendschein

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33 Scopus citations

Abstract

To determine whether myocardial reperfusion can be detected promptly by changes in profiles of isoforms of MM-creatine kinase (CK) in plasma, coronary occlusion was induced in 30 conscious dogs and reperfusion was initiated after 1, 2, 3, or 4 hr in 21. The myocardial isoform of MM-CK, MM(A), was quantified in serial plasma samples by chromatofocusing. Before coronary occlusion, MM(A) comprised 13 ± 7% (SD) of the total CK activity in plasma. The percentage of MM(A) (MM(A)%) was elevated before reperfusion, but increased markedly and consistently to a peak of 52 ± 13% (n = 21) between 30 min and 1 hr after the time of onset of reperfusion. The rate of increase in MM(A)% was significantly faster with reperfusion at 1 hr (1.44 ± 0.42% min-1), 2 hr (1.28 ± 0.45% min-1), or 3 hr (1.02 ± 0.27% min-1) (p<.001), but not with reperfusion at 4 hr (0.48 ± 0.34% min-1) compared with the rate in nonreperfused control dogs (0.29 ± 0.09% min-1). Furthermore, the rate of increase in MM(A)% was neither influenced by peak total CK activity (r = -.1) nor dependent on infarct size measured histochemically 24 hr after coronary occlusion (r = -.003). The time form coronary occlusion to the peak of MM(A)% was reduced by reperfusion at 1 to 3 hr compared with control, but this index was not identified as rapidly as the rate of increase in MM(A)%. Accordingly, characterization of the rate of increase in MM(A)% in plasma when reperfusion occurs early after the onset of myocardial infarction permits prompt, reliable, and noninvasive detection of myocardial reperfusion.

Original languageEnglish
Pages (from-to)567-572
Number of pages6
JournalCirculation
Volume74
Issue number3
DOIs
StatePublished - 1986

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