E2F is required to prevent inappropriate S-phase entry of mammalian cells

Song He, Brian L. Cook, Benjamin E. Deverman, Ulrich Weihe, Fan Zhang, Vivek Prachand, Jie Zheng, Steven J. Weintraub

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

E2F is a family of transcription factors that regulates the cell cycle. It is widely accepted that E2F-mediated transactivation of a set of genes is the critical activity that governs cellular progression through G1 into S phase. In contrast to this hypothesis, we demonstrate that E2F actually suppresses the onset of S phase in two cell types when the cells are arrested by gamma irradiation. Our findings indicate that in these cells, the critical event triggering progression from G0/G1 arrest into S phase is the release of E2F-mediated transrepression of cell cycle genes, not transactivation by E2F. Furthermore, our data suggest that E2F-mediated transactivation is not necessary for the G1/S-phase transition in these cells.

Original languageEnglish
Pages (from-to)363-371
Number of pages9
JournalMolecular and cellular biology
Volume20
Issue number1
DOIs
StatePublished - Jan 2000

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