Dynamic regulation of c-Myc proto-oncogene expression during lymphocyte development revealed by a GFP-c-Myc knock-in mouse

Ching Yu Huang, Andrea L. Bredemeyer, Laura M. Walker, Craig H. Bassing, Barry P. Sleckman

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

c-Myc induces widely varying cellular effects, including cell proliferation and cell death. These different cellular effects are determined, in part, by c-Myc protein expression levels, which are regulated through several transcriptional and post-transcriptional pathways. c-Myc transcripts can be detected in cells at all stages of B and T lymphocyte development. However, little is known about c-Myc protein expression, and how it varies, in developing lymphocytes. Here mice have been generated in which the endogenous c-Myc locus has been modified (c-MycG) so that it encodes a GFP-c-Myc fusion protein. c-MycG/G mice are viable, appear normal and exhibit grossly normal lymphocyte development. Flow cytometric analyses revealed significant heterogeneity in c-Myc protein expression levels in developing c-MycG/ G B and T lymphocytes. GFP-c-Myc expression levels were highest in proliferating lymphocytes, suggesting that c-Myc up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.

Original languageEnglish
Pages (from-to)342-349
Number of pages8
JournalEuropean Journal of Immunology
Volume38
Issue number2
DOIs
StatePublished - Feb 1 2008

Keywords

  • Cell cycle
  • Development
  • Lymphocyte
  • Oncogene
  • c-Myc

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