Dynamic contrast optical coherence tomography (DyC-OCT) for label-free live cell imaging

Chao Ren; Senyue Hao; Fei Wang; Abigail Matt; Marcello Magri Amaral; Daniel Yang; Leyao Wang; Chao Zhou

doi:10.1038/s42003-024-05973-5

Dynamic contrast optical coherence tomography (DyC-OCT) for label-free live cell imaging

Chao Ren
, Senyue Hao
, Fei Wang
, Abigail Matt
, Marcello Magri Amaral
, Daniel Yang
, Leyao Wang
, Chao Zhou

Research output: Contribution to journal › Short survey › peer-review

37 Scopus citations

Abstract

Dynamic contrast optical coherence tomography (DyC-OCT), an emerging imaging method, utilizes fluctuation patterns in OCT signals to enhance contrast, thereby enabling non-invasive label-free volumetric live cell imaging. In this mini review, we explain the core concepts behind DyC-OCT image formation and its system configurations, serving as practical guidance for future DyC-OCT users. Subsequently, we explore its applications in delivering high-quality, contrast-enhanced images of cellular morphology, as well as in monitoring changes in cellular activity/viability assay experiments.

Original language	English
Article number	278
Journal	Communications Biology
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s42003-024-05973-5
State	Published - Dec 2024

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title = "Dynamic contrast optical coherence tomography (DyC-OCT) for label-free live cell imaging",

abstract = "Dynamic contrast optical coherence tomography (DyC-OCT), an emerging imaging method, utilizes fluctuation patterns in OCT signals to enhance contrast, thereby enabling non-invasive label-free volumetric live cell imaging. In this mini review, we explain the core concepts behind DyC-OCT image formation and its system configurations, serving as practical guidance for future DyC-OCT users. Subsequently, we explore its applications in delivering high-quality, contrast-enhanced images of cellular morphology, as well as in monitoring changes in cellular activity/viability assay experiments.",

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AU - Ren, Chao

AU - Hao, Senyue

AU - Wang, Fei

AU - Matt, Abigail

AU - Amaral, Marcello Magri

AU - Yang, Daniel

AU - Wang, Leyao

AU - Zhou, Chao

PY - 2024/12

Y1 - 2024/12

N2 - Dynamic contrast optical coherence tomography (DyC-OCT), an emerging imaging method, utilizes fluctuation patterns in OCT signals to enhance contrast, thereby enabling non-invasive label-free volumetric live cell imaging. In this mini review, we explain the core concepts behind DyC-OCT image formation and its system configurations, serving as practical guidance for future DyC-OCT users. Subsequently, we explore its applications in delivering high-quality, contrast-enhanced images of cellular morphology, as well as in monitoring changes in cellular activity/viability assay experiments.

AB - Dynamic contrast optical coherence tomography (DyC-OCT), an emerging imaging method, utilizes fluctuation patterns in OCT signals to enhance contrast, thereby enabling non-invasive label-free volumetric live cell imaging. In this mini review, we explain the core concepts behind DyC-OCT image formation and its system configurations, serving as practical guidance for future DyC-OCT users. Subsequently, we explore its applications in delivering high-quality, contrast-enhanced images of cellular morphology, as well as in monitoring changes in cellular activity/viability assay experiments.

UR - https://www.scopus.com/pages/publications/85187164968

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DO - 10.1038/s42003-024-05973-5

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JF - Communications Biology

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ER -

Dynamic contrast optical coherence tomography (DyC-OCT) for label-free live cell imaging

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