Dynamic changes in the transcriptome and methylome of Chlamydomonas reinhardtii throughout its life cycle

David Lopez, Takashi Hamaji, Janette Kropat, Peter De Hoff, Marco Morselli, Liudmilla Rubbi, Sorel Fitz-Gibbon, Sean D. Gallaher, Sabeeha S. Merchant, James Umen, Matteo Pellegrini

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

The green alga Chlamydomonas reinhardtii undergoes gametogenesis and mating upon nitrogen starvation. While the steps involved in its sexual reproductive cycle have been extensively characterized, the genome-wide transcriptional and epigenetic changes underlying different life cycle stages have yet to be fully described. Here, we performed transcriptome and methylome sequencing to quantify expression and DNA methylation from vegetative and gametic cells of each mating type and from zygotes. We identified 361 gametic genes with mating type-specific expression patterns and 627 genes that are specifically induced in zygotes; furthermore, these sex-related gene sets were enriched for secretory pathway and alga-specific genes. We also examined the C. reinhardtii nuclear methylation map with base-level resolution at different life cycle stages. Despite having low global levels of nuclear methylation, we detected 23 hypermethylated loci in gene-poor, repeat-rich regions. We observed mating type-specific differences in chloroplast DNA methylation levels in plus versus minus mating type gametes followed by chloroplast DNA hypermethylation in zygotes. Lastly, we examined the expression of candidate DNA methyl transferases and found three, DMT1a, DMT1b, and DMT4, that are differentially expressed during the life cycle and are candidate DNA methylases. The expression and methylation data we present provide insight into cell type-specific transcriptional and epigenetic programs during key stages of the C. reinhardtii life cycle.

Original languageEnglish
Pages (from-to)2730-2743
Number of pages14
JournalPlant Physiology
Volume169
Issue number4
DOIs
StatePublished - Dec 2015

Fingerprint

Dive into the research topics of 'Dynamic changes in the transcriptome and methylome of Chlamydomonas reinhardtii throughout its life cycle'. Together they form a unique fingerprint.

Cite this