Dynamic changes in RNA–protein interactions and RNA secondary structure in mammalian erythropoiesis

Mengge Shan, Xinjun Ji, Kevin Janssen, Ian M. Silverman, Jesse Humenik, Ben A. Garcia, Stephen A. Liebhaber, Brian D. Gregory

Research output: Contribution to journalArticlepeer-review

Abstract

Two features of eukaryotic RNA molecules that regulate their post-transcriptional fates are RNA secondary structure and RNA-binding protein (RBP) interaction sites. However, a comprehensive global overview of the dynamic nature of these sequence features during erythropoiesis has never been obtained. Here, we use our ribonuclease-mediated structure and RBP-binding site mapping approach to reveal the global landscape of RNA secondary structure and RBP–RNA interaction sites and the dynamics of these features during this important developmental process. We identify dynamic patterns of RNA secondary structure and RBP binding throughout the process and determine a set of corresponding protein-bound sequence motifs along with their dynamic structural and RBP-binding contexts. Finally, using these dynamically bound sequences, we identify a number of RBPs that have known and putative key functions in post-transcriptional regulation during mammalian erythropoiesis. In total, this global analysis reveals new post-transcriptional regulators of mammalian blood cell development.

Original languageEnglish
Article numbere202000659
JournalLife Science Alliance
Volume4
Issue number9
DOIs
StatePublished - Sep 2021

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