Dynamic assessment of bilayer thickness by varying phospholipid and hydraphile synthetic channel chain lengths

Michelle E. Weber, Paul H. Schlesinger, George W. Gokel

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

A library of "hydraphile" synthetic ion channel analogues that differ in overall length from ∼28-58 Å has been prepared. A new and convenient ion-selective electrode (ISE) method was used to assay Na+ release. Liposomes were formed from three different phospholipids: 1,2-dimyristoleoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dioleoyl-sn-glycero- 3-phosphocholine (DOPC), and 1,2-dierucoyl-sn-glycero-3-phosphocholine (DEPC). The acyl chains of the lipids comprise cis-unsaturated 14:1, 18:1, or 22:1 residues, respectively. Sodium release was measured for each liposome system with each of the synthetic channels. Peak activity was observed for shorter channels in liposomes formed from DMPC and for longer channels in DEPC. A separate study was then conducted in DMPC liposomes in the presence of the putative membrane-thickening agents cholesterol and decane. Peak activity was clearly shifted to longer channel lengths upon addition of 20 or 40 mol % cholesterol or n-decane to the liposome preparation.

Original languageEnglish
Pages (from-to)636-642
Number of pages7
JournalJournal of the American Chemical Society
Volume127
Issue number2
DOIs
StatePublished - Jan 19 2005

Fingerprint Dive into the research topics of 'Dynamic assessment of bilayer thickness by varying phospholipid and hydraphile synthetic channel chain lengths'. Together they form a unique fingerprint.

  • Cite this