TY - JOUR
T1 - Dynamic and electrokinetic behavior of erythrocyte membrane in diabetes mellitus and diabetic cardiovascular disease
AU - Adak, Sangeeta
AU - Chowdhury, Subhankar
AU - Bhattacharyya, Maitree
N1 - Funding Information:
We acknowledge Dr. Philip S. Lows, Department of Chemistry, Purdue University, West Lafayette, Indiana for providing us with the polyclonal rabbit anti-ankyrin antibody. We are grateful to Dr. Sanjay Ghosh and his research group, Department of Biochemistry, University of Calcutta for their support in performing Western blot experiments. We thank Prof. Anjan Das Gupta, Department of Biochemistry, Calcutta University for providing the facility of Easy Gel Output Software (version 1.1) for Densitometric Scanning. We express our sincere indebtedness to Prof. Kalyan Das, Department of Statistics, University of Calcutta for his suggestions regarding the statistical data analysis. Financial support from ICMR (Indian Council of Medical Research), and instrumental facility provided by DST (FIST) India is also gratefully acknowledged.
PY - 2008/2
Y1 - 2008/2
N2 - The dynamic and electrokinetic properties of erythrocyte membrane are explored as significant indices involved in the association of diabetes and diabetic cardiovascular disease. Lipid peroxidation studies reveal malondialdehyde concentration to reach a maximum in diabetic cardiovascular patients. Lower fluidity of erythrocyte membrane implies declined ability of erythrocyte to deform in pathogenic state, which is supported by decreased osmotic resistance. Membrane protein profile modification detected by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) indicates a significant reduction in the quantity of ankyrin protein band 2.1 in diabetic subjects. In addition the reduction in an immunoreactive band against polyclonal anti-ankyrin antibody during Western blot analysis confirms the modification of ankyrin protein in diseased erythrocyte (reported for the first time). The electrokinetic behavior of erythrocyte membrane is monitored by laser Doppler velocimetry mode of the Nano-ZS. Changes in zeta potential values of the red blood cell membrane are consistent with decreased membrane fluidity in diseased erythrocytes (reported for the first time). Membrane potential values of control, diabetic and diabetic cardiovascular erythrocytes are - 37.24 ± 1.5 mV, - 28.44 ± 1.34 mV, and - 22.21 ± 1.21 mV respectively indicating a gradual lowering of zeta potential when erythrocyte membrane undergoes progressive changes - from simple agglomeration to fluid gel formation - and finally to a rigid gel.
AB - The dynamic and electrokinetic properties of erythrocyte membrane are explored as significant indices involved in the association of diabetes and diabetic cardiovascular disease. Lipid peroxidation studies reveal malondialdehyde concentration to reach a maximum in diabetic cardiovascular patients. Lower fluidity of erythrocyte membrane implies declined ability of erythrocyte to deform in pathogenic state, which is supported by decreased osmotic resistance. Membrane protein profile modification detected by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) indicates a significant reduction in the quantity of ankyrin protein band 2.1 in diabetic subjects. In addition the reduction in an immunoreactive band against polyclonal anti-ankyrin antibody during Western blot analysis confirms the modification of ankyrin protein in diseased erythrocyte (reported for the first time). The electrokinetic behavior of erythrocyte membrane is monitored by laser Doppler velocimetry mode of the Nano-ZS. Changes in zeta potential values of the red blood cell membrane are consistent with decreased membrane fluidity in diseased erythrocytes (reported for the first time). Membrane potential values of control, diabetic and diabetic cardiovascular erythrocytes are - 37.24 ± 1.5 mV, - 28.44 ± 1.34 mV, and - 22.21 ± 1.21 mV respectively indicating a gradual lowering of zeta potential when erythrocyte membrane undergoes progressive changes - from simple agglomeration to fluid gel formation - and finally to a rigid gel.
KW - Ankyrin
KW - Cardiovascular disease
KW - Hyperglycemia
KW - Membrane fluidity
KW - Oxidative stress
KW - Zeta potential
UR - http://www.scopus.com/inward/record.url?scp=38749086003&partnerID=8YFLogxK
U2 - 10.1016/j.bbagen.2007.10.013
DO - 10.1016/j.bbagen.2007.10.013
M3 - Article
C2 - 18035063
AN - SCOPUS:38749086003
VL - 1780
SP - 108
EP - 115
JO - BBA - General Subjects
JF - BBA - General Subjects
SN - 0304-4165
IS - 2
ER -