@inproceedings{e48ce4db422c4306b412b04998f89240,
title = "Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation",
abstract = "Quantitative and noninvasive measurement of protease activities has remained an imaging challenge in deep tissues such as the lungs. Here, we designed a dual-radiolabeled probe for reporting the activities of proteases such as matrix metalloproteinases (MMPs) with multispectral single photon emission computed tomography (SPECT) imaging. A gold nanoparticle (NP) was radiolabeled with 125I and 111In and functionalized with an MMP9-cleavable peptide to form a multispectral SPECT imaging contrast agent. In another design, incorporation of 199Au radionuclide into the metal crystal structure of gold NPs provided a superior and stable reference signal in lungs, and 111In was linked to the NP surface via a protease-cleavable substrate, which can serve as an enzyme activity reporter. This work reveals strategies to correlate protease activities with diverse pathologies in a tissue-depth independent manner.",
keywords = "MMP, SPECT, bioimaging, enzyme, in vivo imaging, nanoparticles",
author = "Black, {Kvar C.L.} and Mingzhou Zhou and Pinaki Sarder and Maryna Kuchuk and Al-Yasiri, {Amal Y.} and Gunsten, {Sean P.} and Kexian Liang and Hennkens, {Heather M.} and Akers, {Walter J.} and Richard Laforest and Brody, {Steven L.} and Cutler, {Cathy S.} and Samuel Achilefu",
note = "Funding Information: This work was supported in part by grants from the US National Institutes of Health (NCI - U54 CA199092, R01 CA194552, R01 CA152329, P50 CA094056; NHLBI - HHSN268201000046C; NIBIB - R01 EB008111, R01 EB021048), the Department of Defense Breast Cancer Research Program (W81XWH-16-1-0286), and the Alvin J. Siteman Cancer Research Fund (11-FY16-01). Al-Yasiri was supported by the University of Baghdad. We thank the Missouri University Research Reactor for the 199Au synthesis and generously providing the 199Au gold nanocrystal suspensions. We acknowledge Gail Sudlow and LeMoyne Habimana-Griffin for experimental assistance. Publisher Copyright: {\textcopyright} 2018 SPIE.; Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X 2018 ; Conference date: 29-01-2018 Through 30-01-2018",
year = "2018",
doi = "10.1117/12.2301033",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Samuel Achilefu and Ramesh Raghavachari",
booktitle = "Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X",
}