@inproceedings{e48ce4db422c4306b412b04998f89240,
title = "Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation",
abstract = "Quantitative and noninvasive measurement of protease activities has remained an imaging challenge in deep tissues such as the lungs. Here, we designed a dual-radiolabeled probe for reporting the activities of proteases such as matrix metalloproteinases (MMPs) with multispectral single photon emission computed tomography (SPECT) imaging. A gold nanoparticle (NP) was radiolabeled with 125I and 111In and functionalized with an MMP9-cleavable peptide to form a multispectral SPECT imaging contrast agent. In another design, incorporation of 199Au radionuclide into the metal crystal structure of gold NPs provided a superior and stable reference signal in lungs, and 111In was linked to the NP surface via a protease-cleavable substrate, which can serve as an enzyme activity reporter. This work reveals strategies to correlate protease activities with diverse pathologies in a tissue-depth independent manner.",
keywords = "MMP, SPECT, bioimaging, enzyme, in vivo imaging, nanoparticles",
author = "Black, {Kvar C.L.} and Mingzhou Zhou and Pinaki Sarder and Maryna Kuchuk and Al-Yasiri, {Amal Y.} and Gunsten, {Sean P.} and Kexian Liang and Hennkens, {Heather M.} and Akers, {Walter J.} and Richard Laforest and Brody, {Steven L.} and Cutler, {Cathy S.} and Samuel Achilefu",
note = "Publisher Copyright: {\textcopyright} 2018 SPIE.; Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X 2018 ; Conference date: 29-01-2018 Through 30-01-2018",
year = "2018",
doi = "10.1117/12.2301033",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Samuel Achilefu and Ramesh Raghavachari",
booktitle = "Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X",
}