TY - JOUR
T1 - Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types
AU - Kannan, Madhuvanthi
AU - Vasan, Ganesh
AU - Haziza, Simon
AU - Huang, Cheng
AU - Chrapkiewicz, Radosław
AU - Luo, Junjie
AU - Cardin, Jessica A.
AU - Schnitzer, Mark J.
AU - Pieribone, Vincent A.
N1 - Publisher Copyright:
Copyright © 2022 The Authors, some rights reserved.
PY - 2022/11/4
Y1 - 2022/11/4
N2 - Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between targeted cell populations. However, current indicators lack the requisite sensitivity for in vivo multipopulation imaging. We describe next-generation green and red voltage sensors, Ace-mNeon2 and VARNAM2, and their reverse response-polarity variants pAce and pAceR. Our indicators enable 0.4- to 1-kilohertz voltage recordings from >50 spiking neurons per field of view in awake mice and ~30-minute continuous imaging in flies. Using dual-polarity multiplexed imaging, we uncovered brain state–dependent antagonism between neocortical somatostatin-expressing (SST+) and vasoactive intestinal peptide–expressing (VIP+) interneurons and contributions to hippocampal field potentials from cell ensembles with distinct axonal projections. By combining three mutually compatible indicators, we performed simultaneous triple-population imaging. These approaches will empower investigations of the dynamic interplay between neuronal subclasses at single-spike resolution.
AB - Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between targeted cell populations. However, current indicators lack the requisite sensitivity for in vivo multipopulation imaging. We describe next-generation green and red voltage sensors, Ace-mNeon2 and VARNAM2, and their reverse response-polarity variants pAce and pAceR. Our indicators enable 0.4- to 1-kilohertz voltage recordings from >50 spiking neurons per field of view in awake mice and ~30-minute continuous imaging in flies. Using dual-polarity multiplexed imaging, we uncovered brain state–dependent antagonism between neocortical somatostatin-expressing (SST+) and vasoactive intestinal peptide–expressing (VIP+) interneurons and contributions to hippocampal field potentials from cell ensembles with distinct axonal projections. By combining three mutually compatible indicators, we performed simultaneous triple-population imaging. These approaches will empower investigations of the dynamic interplay between neuronal subclasses at single-spike resolution.
UR - http://www.scopus.com/inward/record.url?scp=85141943012&partnerID=8YFLogxK
U2 - 10.1126/science.abm8797
DO - 10.1126/science.abm8797
M3 - Article
C2 - 36378956
AN - SCOPUS:85141943012
SN - 0036-8075
VL - 378
JO - Science
JF - Science
IS - 6619
M1 - eabm8797
ER -