Drosophila RNAi screen reveals CD36 family member required for mycobacterial infection

Jennifer A. Philips, Eric J. Rubin, Norbert Perrimon

Research output: Contribution to journalArticlepeer-review

299 Scopus citations

Abstract

Certain pathogens, such as Mycobacterium tuberculosis, survive within the hostile intracellular environment of a macrophage. To identify host factors required for mycobacterial entry and survival within macrophages, we performed a genome-wide RNA interference screen in Drosophila macrophage-like cells, using Mycobacterium fortuitum. We identified factors required for general phagocytosis, as well as those needed specifically for mycobacterial infection. One specific factor. Peste (Pes), is a CD36 family member required for uptake of mycobacteria, but not Escherichia coli or Staphylococcus aureus. Moreover, mammalian class B scavenger receptors (SRs) conferred uptake of bacteria into nonphagocytic cells, with SR-BI and SR-BII uniquely mediating uptake of M. fortuitum, which suggests a conserved role for class B SRs in pattern recognition and innate immunity.

Original languageEnglish
Pages (from-to)1251-1253
Number of pages3
JournalScience
Volume309
Issue number5738
DOIs
StatePublished - Aug 19 2005

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