TY - JOUR
T1 - Droplet-based single-cell joint profiling of histone modifications and transcriptomes
AU - Xie, Yang
AU - Zhu, Chenxu
AU - Wang, Zhaoning
AU - Tastemel, Melodi
AU - Chang, Lei
AU - Li, Yang Eric
AU - Ren, Bing
N1 - Publisher Copyright:
© 2023, The Author(s).
PY - 2023/10
Y1 - 2023/10
N2 - We previously reported Paired-Tag, a combinatorial indexing-based method that can simultaneously map histone modifications and gene expression at single-cell resolution at scale. However, the lengthy procedure of Paired-Tag has hindered its general adoption in the community. To address this bottleneck, we developed a droplet-based Paired-Tag protocol that is faster and more accessible than the previous method. Using cultured mammalian cells and primary brain tissues, we demonstrate its superior performance at identifying candidate cis-regulatory elements and associating their dynamic chromatin state to target gene expression in each constituent cell type in a complex tissue.
AB - We previously reported Paired-Tag, a combinatorial indexing-based method that can simultaneously map histone modifications and gene expression at single-cell resolution at scale. However, the lengthy procedure of Paired-Tag has hindered its general adoption in the community. To address this bottleneck, we developed a droplet-based Paired-Tag protocol that is faster and more accessible than the previous method. Using cultured mammalian cells and primary brain tissues, we demonstrate its superior performance at identifying candidate cis-regulatory elements and associating their dynamic chromatin state to target gene expression in each constituent cell type in a complex tissue.
UR - http://www.scopus.com/inward/record.url?scp=85167511508&partnerID=8YFLogxK
U2 - 10.1038/s41594-023-01060-1
DO - 10.1038/s41594-023-01060-1
M3 - Article
C2 - 37563440
AN - SCOPUS:85167511508
SN - 1545-9993
VL - 30
SP - 1428
EP - 1433
JO - Nature Structural and Molecular Biology
JF - Nature Structural and Molecular Biology
IS - 10
ER -