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Drawbacks in the use of unconventional hydrophobic anhydrides for histone derivatization in bottom-up proteomics PTM analysis
Simone Sidoli
, Zuo Fei Yuan
, Shu Lin
, Kelly Karch
, Xiaoshi Wang
, Natarajan Bhanu
, Anna M. Arnaudo
, Laura Mae Britton
, Xing Jun Cao
, Michelle Gonzales-Cope
, Yumiao Han
, Shichong Liu
, Rosalynn C. Molden
, Samuel Wein
, Leila Afjehi-Sadat
,
Benjamin A. Garcia
School of Medicine
Department of Biochemistry & Molecular Biophysics
Siteman Cancer Center
Center for Drug Discovery
Roy and Diana Vagelos Division of Biology & Biomedical Sciences (DBBS)
Brain Tumor Center
DBBS - Biochemistry, Biophysics, and Structural Biology
Research output
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Contribution to journal
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Article
›
peer-review
36
Scopus citations
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Keyphrases
Histone
100%
Derivatization
100%
Bottom-up Proteomics
100%
Anhydride
100%
PTM Analysis
100%
MS-MS
50%
Retention Time
50%
Histone Peptides
50%
Propionic Anhydride
50%
Lysine Residue
25%
Cell Lysate
25%
Commercially Available
25%
Lysine
25%
Ionization Efficiency
25%
MS-based Proteomics
25%
Reversed-phase HPLC
25%
Hydrophobicity
25%
Trypsin Digestion
25%
Short Peptides
25%
Arginine Residue
25%
Histone H3 Tail
25%
MS Spectra
25%
Histone PTMs
25%
Peptide Retention
25%
Propionylation
25%
Propionic
25%
Fragmentation Efficiency
25%
Spectral Quality
25%
Benzoic
25%
MS Fragmentation
25%
Chemistry
Derivatization
100%
Anhydride
100%
Histone
100%
Proteomics
100%
Tandem Mass Spectrometry
28%
Retention Time
28%
Lysine
28%
Hydrophobicity
14%
Propionyl Group
14%
Arginine
14%
Allopurinol
14%