Down-regulation of the kps region 1 capsular assembly operon following attachment of Escherichia coli type 1 fimbriae to D-mannose receptors

William R. Schwan, Michael T. Beck, Scott J. Hultgren, Jerry Pinkner, Nathan L. Woolever, Thomas Larson

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A differential-display PCR procedure identified the capsular assembly gene kpsD after Escherichia coli type 1 fimbrial binding to mannose-coated Sepharose beads. Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of the kpsD gene, and Northern blot and lacZ fusion analyses showed down-regulation of the kpsFEDUCS region 1 operon. KpsD protein levels fell, and an agglutination test showed less K capsular antigen on the surface following the bacterial ligand-receptor interaction. These data show that binding of type 1 fimbriae (pili) to D-mannose receptors triggers a cross talk that leads to down-regulation of the capsule assembly region 1 operon in uropathogenic E. coli.

Original languageEnglish
Pages (from-to)1226-1231
Number of pages6
JournalInfection and immunity
Volume73
Issue number2
DOIs
StatePublished - Feb 1 2005

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