Double-illumination photoacoustic microscopy of intestinal hemodynamics following massive small bowel resection

Junjie Yao, Kathryn J. Rowland, Lidai Wang, Konstantin I. Maslov, Brad W. Warner, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Massive small bowel resection (SBR) results in villus angiogenesis and intestinal adaptation. The exact mechanism that causes intestinal villus angiogenesis remains unknown. We hypothesize that hemodynamic changes within the remnant bowel after SBR will trigger intestinal angiogenesis. To validate this, we used photoacoustic microscopy (PAM) to image the microvascular system of the intestine in C57B6 mice and to measure blood flow and oxygen saturation (sO 2) of a supplying artery and vein. Baseline measurements were made 6 cm proximal to the ileal-cecal junction (ICJ) prior to resection. A 50% proximal bowel resection was then performed, and measurements were again recorded at the same location immediately, 1, 3 and 7 days following resection. The results show that arterial and venous sO 2 were similar prior to SBR. Immediately following SBR, the arterial and venous sO 2 decreased by 14.3 ± 2.7% and 32.7 ± 6.6%, respectively, while the arterial and venous flow speed decreased by 62.9 ± 17.3% and 60.0 ± 20.1%, respectively. Such significant decreases in sO 2 and blood flow indicate a hypoxic state after SBR. Within one week after SBR, both sO 2 and blood flow speed had gradually recovered. By 7 days after SBR, arterial and venous sO 2 had increased to 101.0 ± 2.9% and 82.7 ± 7.3% of the baseline values, respectively, while arterial and venous flow speed had increased to 106.0 ± 21.4% and 150.0 ± 29.6% of the baseline values, respectively. Such increases in sO 2 and blood flow may result from angiogenesis following SBR.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2012
DOIs
StatePublished - Apr 9 2012
EventPhotons Plus Ultrasound: Imaging and Sensing 2012 - San Francisco, CA, United States
Duration: Jan 22 2012Jan 24 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8223
ISSN (Print)1605-7422

Conference

ConferencePhotons Plus Ultrasound: Imaging and Sensing 2012
CountryUnited States
CitySan Francisco, CA
Period01/22/1201/24/12

Keywords

  • Angiogenesis
  • Blood flow
  • Hypoxia
  • Oxygen saturation
  • Photoacoustic microscopy
  • Small bowel resection

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    Yao, J., Rowland, K. J., Wang, L., Maslov, K. I., Warner, B. W., & Wang, L. V. (2012). Double-illumination photoacoustic microscopy of intestinal hemodynamics following massive small bowel resection. In Photons Plus Ultrasound: Imaging and Sensing 2012 [82233V] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8223). https://doi.org/10.1117/12.909508