Double-illumination photoacoustic microscopy

Junjie Yao, Konstantin I. Maslov, Ernest R. Puckett, Kathryn J. Rowland, Brad W. Warner, Lihong V. Wang

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


Recent developments of optical-resolution photoacoustic microscopy (OR-PAM) have improved its spatial resolution and imaging speed. However, the penetration depth of OR-PAM is still limited to ∼1 mm in tissue, owing to the strong tissue scattering. Here, we have developed double-illumination PAM (DI-PAM), which illuminates the sample from both top and bottom sides simultaneously. Through phantom and in vivo experiments, we have demonstrated for thin targets that DI-PAM has a penetration depth of ∼2 mm in tissue at 532 nm and a focal zone of 260 μm, both significant improvements over traditional reflection or transmission-mode OR-PAM.

Original languageEnglish
Pages (from-to)659-661
Number of pages3
JournalOptics Letters
Issue number4
StatePublished - Feb 15 2012


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