Double-illumination photoacoustic microscopy

Junjie Yao; Konstantin I. Maslov; Ernest R. Puckett; Kathryn J. Rowland; Brad W. Warner; Lihong V. Wang

doi:10.1364/OL.37.000659

Double-illumination photoacoustic microscopy

Junjie Yao, Konstantin I. Maslov, Ernest R. Puckett, Kathryn J. Rowland, Brad W. Warner, Lihong V. Wang

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Recent developments of optical-resolution photoacoustic microscopy (OR-PAM) have improved its spatial resolution and imaging speed. However, the penetration depth of OR-PAM is still limited to ∼1 mm in tissue, owing to the strong tissue scattering. Here, we have developed double-illumination PAM (DI-PAM), which illuminates the sample from both top and bottom sides simultaneously. Through phantom and in vivo experiments, we have demonstrated for thin targets that DI-PAM has a penetration depth of ∼2 mm in tissue at 532 nm and a focal zone of 260 μm, both significant improvements over traditional reflection or transmission-mode OR-PAM.

Original language	English
Pages (from-to)	659-661
Number of pages	3
Journal	Optics Letters
Volume	37
Issue number	4
DOIs	https://doi.org/10.1364/OL.37.000659
State	Published - Feb 15 2012

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title = "Double-illumination photoacoustic microscopy",

abstract = "Recent developments of optical-resolution photoacoustic microscopy (OR-PAM) have improved its spatial resolution and imaging speed. However, the penetration depth of OR-PAM is still limited to ∼1 mm in tissue, owing to the strong tissue scattering. Here, we have developed double-illumination PAM (DI-PAM), which illuminates the sample from both top and bottom sides simultaneously. Through phantom and in vivo experiments, we have demonstrated for thin targets that DI-PAM has a penetration depth of ∼2 mm in tissue at 532 nm and a focal zone of 260 μm, both significant improvements over traditional reflection or transmission-mode OR-PAM.",

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AU - Yao, Junjie

AU - Maslov, Konstantin I.

AU - Puckett, Ernest R.

AU - Rowland, Kathryn J.

AU - Warner, Brad W.

AU - Wang, Lihong V.

PY - 2012/2/15

Y1 - 2012/2/15

N2 - Recent developments of optical-resolution photoacoustic microscopy (OR-PAM) have improved its spatial resolution and imaging speed. However, the penetration depth of OR-PAM is still limited to ∼1 mm in tissue, owing to the strong tissue scattering. Here, we have developed double-illumination PAM (DI-PAM), which illuminates the sample from both top and bottom sides simultaneously. Through phantom and in vivo experiments, we have demonstrated for thin targets that DI-PAM has a penetration depth of ∼2 mm in tissue at 532 nm and a focal zone of 260 μm, both significant improvements over traditional reflection or transmission-mode OR-PAM.

AB - Recent developments of optical-resolution photoacoustic microscopy (OR-PAM) have improved its spatial resolution and imaging speed. However, the penetration depth of OR-PAM is still limited to ∼1 mm in tissue, owing to the strong tissue scattering. Here, we have developed double-illumination PAM (DI-PAM), which illuminates the sample from both top and bottom sides simultaneously. Through phantom and in vivo experiments, we have demonstrated for thin targets that DI-PAM has a penetration depth of ∼2 mm in tissue at 532 nm and a focal zone of 260 μm, both significant improvements over traditional reflection or transmission-mode OR-PAM.

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VL - 37

SP - 659

EP - 661

JO - Optics Letters

JF - Optics Letters

IS - 4

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Double-illumination photoacoustic microscopy

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