Dot-based ELISA and RIA: Two rapid assays that screen hybridoma supernatants against whole live cells

M. Stya; R. Wahl; W. H. Beierwaltes

doi:10.1016/0022-1759(84)90033-4

Dot-based ELISA and RIA: Two rapid assays that screen hybridoma supernatants against whole live cells

M. Stya
, R. Wahl
, W. H. Beierwaltes

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Two assays are described that are suitable for the screening of large numbers of hybridoma supernatants as well as for the screening of a wide range of tissues for the distribution of a given cell surface antigen. These assays use whole live cells as targets, avoiding fixation or extraction, which could alter the antigenic structural profile. The assays are rapid, simple and inexpensive. Their advantages and applications are discussed.

Original language	English
Pages (from-to)	75-81
Number of pages	7
Journal	Journal of Immunological Methods
Volume	73
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(84)90033-4
State	Published - Oct 12 1984

Keywords

ELISA
hybridoma screening
radioimmunoassay

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10.1016/0022-1759(84)90033-4

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title = "Dot-based ELISA and RIA: Two rapid assays that screen hybridoma supernatants against whole live cells",

abstract = "Two assays are described that are suitable for the screening of large numbers of hybridoma supernatants as well as for the screening of a wide range of tissues for the distribution of a given cell surface antigen. These assays use whole live cells as targets, avoiding fixation or extraction, which could alter the antigenic structural profile. The assays are rapid, simple and inexpensive. Their advantages and applications are discussed.",

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AU - Stya, M.

AU - Wahl, R.

AU - Beierwaltes, W. H.

PY - 1984/10/12

Y1 - 1984/10/12

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AB - Two assays are described that are suitable for the screening of large numbers of hybridoma supernatants as well as for the screening of a wide range of tissues for the distribution of a given cell surface antigen. These assays use whole live cells as targets, avoiding fixation or extraction, which could alter the antigenic structural profile. The assays are rapid, simple and inexpensive. Their advantages and applications are discussed.

KW - ELISA

KW - hybridoma screening

KW - radioimmunoassay

UR - https://www.scopus.com/pages/publications/0021157864

U2 - 10.1016/0022-1759(84)90033-4

DO - 10.1016/0022-1759(84)90033-4

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AN - SCOPUS:0021157864

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Dot-based ELISA and RIA: Two rapid assays that screen hybridoma supernatants against whole live cells

Abstract

Keywords

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