Dot-based ELISA and RIA: Two rapid assays that screen hybridoma supernatants against whole live cells

M. Stya; R. Wahl; W. H. Beierwaltes

doi:10.1016/0022-1759(84)90033-4

Dot-based ELISA and RIA: Two rapid assays that screen hybridoma supernatants against whole live cells

M. Stya, R. Wahl, W. H. Beierwaltes

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Abstract

Two assays are described that are suitable for the screening of large numbers of hybridoma supernatants as well as for the screening of a wide range of tissues for the distribution of a given cell surface antigen. These assays use whole live cells as targets, avoiding fixation or extraction, which could alter the antigenic structural profile. The assays are rapid, simple and inexpensive. Their advantages and applications are discussed.

Original language	English
Pages (from-to)	75-81
Number of pages	7
Journal	Journal of Immunological Methods
Volume	73
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(84)90033-4
State	Published - Oct 12 1984

Keywords

ELISA
hybridoma screening
radioimmunoassay

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10.1016/0022-1759(84)90033-4

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abstract = "Two assays are described that are suitable for the screening of large numbers of hybridoma supernatants as well as for the screening of a wide range of tissues for the distribution of a given cell surface antigen. These assays use whole live cells as targets, avoiding fixation or extraction, which could alter the antigenic structural profile. The assays are rapid, simple and inexpensive. Their advantages and applications are discussed.",

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KW - radioimmunoassay

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Dot-based ELISA and RIA: Two rapid assays that screen hybridoma supernatants against whole live cells

Abstract

Keywords

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