TY - JOUR
T1 - Dorsal root ganglion neurons expressing trk are selectively sensitive to NGF deprivation in utero
AU - Carroll, Steven L.
AU - Silos-Santiago, Inmaculada
AU - Frese, Susan E.
AU - Ruit, Kenneth G.
AU - Milbrandt, Jeffrey
AU - Snider, William D.
N1 - Funding Information:
We thank E. M. Johnson,Jr., and R. E. Schmidt for comments and suggestions on the manuscript. This work was supported by grants from the National Institutes of Health (ROI NS26224-03 to J. M., and ROI NS-25936 to W. D. S.), the National Science Foundation (BNS 91-12910 to W. D. S.), and the National Multiple Sclerosis Society (RC 1779-B-2 to J. M.). J. M. is an Established Investigator of the American Heart Association (92001820). Support also came from NIH postdoctoral training grants to S. L. C. (5 T32 D DK07296-12) and K. G. R. (NIH NRSA 532 NS-07129). We thank L. Zhang for assistance with some of these experiments.
PY - 1992/10
Y1 - 1992/10
N2 - In utero immune deprivation of the neurotrophic molecule nerve growth factor (NGF) results in the death of most, but not all, mammalian dorsal root ganglion (DRG) neurons. The recent identification of trk, trkB, and trkC as the putative high affinity receptors for NGF, brain-derived neurotrophic factor, and neurotrophin-3, respectively, has allowed an examination of whether their expression by DRG neurons correlates with differential sensitivity to immune deprivation of NGF. In situ hybridization demonstrates that virtually all neurons expressing trk are lost during in utero NGF deprivation. Most, if not all, neurons expressing trkB and trkC survive this treatment. In contrast, the low affinity NGF receptor, p75NGFR, is expressed in both NGF deprivation-resistant and -sensitive neurons. These experiments show that DRG neurons expressing trk require NGF for survival. Futhermore, at least some of the DRG neurons that do not require NGF express the high affinity receptor for another neurotrophin. Finally, these experiments provide evidence that trk, and not p75NGFR, is the primary effector of NGF action in vivo.
AB - In utero immune deprivation of the neurotrophic molecule nerve growth factor (NGF) results in the death of most, but not all, mammalian dorsal root ganglion (DRG) neurons. The recent identification of trk, trkB, and trkC as the putative high affinity receptors for NGF, brain-derived neurotrophic factor, and neurotrophin-3, respectively, has allowed an examination of whether their expression by DRG neurons correlates with differential sensitivity to immune deprivation of NGF. In situ hybridization demonstrates that virtually all neurons expressing trk are lost during in utero NGF deprivation. Most, if not all, neurons expressing trkB and trkC survive this treatment. In contrast, the low affinity NGF receptor, p75NGFR, is expressed in both NGF deprivation-resistant and -sensitive neurons. These experiments show that DRG neurons expressing trk require NGF for survival. Futhermore, at least some of the DRG neurons that do not require NGF express the high affinity receptor for another neurotrophin. Finally, these experiments provide evidence that trk, and not p75NGFR, is the primary effector of NGF action in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0026666339&partnerID=8YFLogxK
U2 - 10.1016/0896-6273(92)90040-K
DO - 10.1016/0896-6273(92)90040-K
M3 - Article
C2 - 1389185
AN - SCOPUS:0026666339
SN - 0896-6273
VL - 9
SP - 779
EP - 788
JO - Neuron
JF - Neuron
IS - 4
ER -