Domain-specific biochemical and serological characterization of SARS-CoV-2 nucleocapsid protein

Chao Wu, Abraham J. Qavi, Austin B. Moyle, Nicole D. Wagner, Asmaa Hachim, Niloufar Kavian, Aidan R. Cole, Joyce Sweeney-Gibbons, Henry W. Rohrs, J. S.Malik Peiris, Christopher F. Basler, Michael L. Gross, Sophie A. Valkenburg, Christopher W. Farnsworth, Gaya K. Amarasinghe, Daisy W. Leung

Research output: Contribution to journalArticlepeer-review


Nucleocapsid proteins are essential for SARS-CoV-2 life cycle. Here, we describe protocols to gather domain-specific insights about essential properties of nucleocapsids. These assays include dynamic light scattering to characterize oligomerization, fluorescence polarization to quantify RNA binding, hydrogen-deuterium exchange mass spectrometry to map RNA binding regions, negative-stain electron microscopy to visualize oligomeric species, interferon reporter assay to evaluate interferon signaling modulation, and a serology assay to reveal insights for improved sensitivity and specificity. These assays are broadly applicable to RNA-encapsidated nucleocapsids. For complete details on the use and execution of this protocol, please refer to Wu et al. (2021).

Original languageEnglish
Article number100906
JournalSTAR Protocols
Issue number4
StatePublished - Dec 17 2021


  • Biophysics
  • Clinical Protocol
  • Health Sciences
  • Immunology
  • Mass Spectrometry
  • Microbiology
  • Microscopy
  • Molecular Biology
  • Protein Biochemistry
  • Protein expression and purification
  • Structural Biology


Dive into the research topics of 'Domain-specific biochemical and serological characterization of SARS-CoV-2 nucleocapsid protein'. Together they form a unique fingerprint.

Cite this