Docosahexaenoic acid conjugated near infrared flourescence probe for in vivo early tumor diagnosis

Siwen Li, Jie Cao, Jingyi Qin, Xin Zhang, Samuel Achilefu, Zhiyu Qian, Yueqing Gu

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Docosahexaenoic acid(DHA) is an omega-3 C22 natural fatty acid with six cis double bonds and as a constituent of membranes used as a precursor for metabolic and biochemical path ways. In this manuscript,we describe the synthesis of near-infrared(NIR) flourescence ICG-Der-01 labeled DHA for in vitro and vivo tumor targeting.The structure of the probe was intensively characterized by UV and MS. The in vitro and vivo tumor targeting abilities of the DHA-based NIR probes were investigeted in MCF-7 cells and MCF-7 xenograft mice model differently by confocal microscopy and CCD camera. The cell cytotoxicity were tested in tumor cells MCF-7.The results shows that the DHA-based NIR probes have high affinity with the tumor both in vitro and vivo.In addition ,we also found that the DHA-based NIR probes have the apparent cytotoxicity on MCF-7 cells.which demonstrated that DHA was conjugated with other antitumor drug could increase the abilities of antirumor efficacy.So DHA-ICG-Der-01 is a promising optical agent for diagnosis of tumors especially in their early stage.

Original languageEnglish
Title of host publicationReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
DOIs
StatePublished - 2013
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V - San Francisco, CA, United States
Duration: Feb 4 2013Feb 6 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8596
ISSN (Print)1605-7422

Conference

ConferenceReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
Country/TerritoryUnited States
CitySan Francisco, CA
Period02/4/1302/6/13

Keywords

  • DHA
  • ICG-Der-01
  • near-infrared
  • tumor targeting

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