DNA transformation activity of a human gastrocarcinoma cell line

Y. Y. Lü; G. R. Lu; X. L. Xu; Z. Ee; J. Miao; H. Li; H. Cai; S. M. Chen; G. R. Deng; P. N. Liu

DNA transformation activity of a human gastrocarcinoma cell line

Y. Y. Lü
, G. R. Lu
, X. L. Xu
, Z. Ee
, J. Miao
, H. Li
, H. Cai
, S. M. Chen
, G. R. Deng
, P. N. Liu

Research output: Contribution to journal › Article › peer-review

Abstract

DNAs of three cell lines of human gastrocarcinoma (MGC-803, BGC-823 and PACM-82) and two fresh solid tumors of human stomach cancer were used to transfect NIH3T3 and Rat-1 cells. The transformed cells were selected with high concentration of glucose and low concentration of serum, or with medium containing Geneticin (G418) after co-transfection of pSVneo and DNAs of stomach cancer cell line or primary transformants. From the second round transfection, we had obtained transformants which could grow with high colony forming efficiency in soft agarose and were tumorigenic in nude mice. The southern blot analysis showed that the cellular DNA of the transformants contained human Alu repeat sequence and the transformed gene from stomach cancer cell line (BGC-823) and was homologous to proto-oncogene c-Ha-ras. The transforming gene is able to induce neoplastic transformation of NIH3T3 and Rat-1 cells.

Original language	English
Pages (from-to)	241-244
Number of pages	4
Journal	Chinese Journal of Oncology
Volume	8
Issue number	4
State	Published - Jul 1986

Cite this

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title = "DNA transformation activity of a human gastrocarcinoma cell line",

abstract = "DNAs of three cell lines of human gastrocarcinoma (MGC-803, BGC-823 and PACM-82) and two fresh solid tumors of human stomach cancer were used to transfect NIH3T3 and Rat-1 cells. The transformed cells were selected with high concentration of glucose and low concentration of serum, or with medium containing Geneticin (G418) after co-transfection of pSVneo and DNAs of stomach cancer cell line or primary transformants. From the second round transfection, we had obtained transformants which could grow with high colony forming efficiency in soft agarose and were tumorigenic in nude mice. The southern blot analysis showed that the cellular DNA of the transformants contained human Alu repeat sequence and the transformed gene from stomach cancer cell line (BGC-823) and was homologous to proto-oncogene c-Ha-ras. The transforming gene is able to induce neoplastic transformation of NIH3T3 and Rat-1 cells.",

author = "L{\"u}, \{Y. Y.\} and Lu, \{G. R.\} and Xu, \{X. L.\} and Z. Ee and J. Miao and H. Li and H. Cai and Chen, \{S. M.\} and Deng, \{G. R.\} and Liu, \{P. N.\}",

year = "1986",

month = jul,

language = "English",

volume = "8",

pages = "241--244",

journal = "Chinese Journal of Oncology",

issn = "0253-3766",

number = "4",

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TY - JOUR

T1 - DNA transformation activity of a human gastrocarcinoma cell line

AU - Lü, Y. Y.

AU - Lu, G. R.

AU - Xu, X. L.

AU - Ee, Z.

AU - Miao, J.

AU - Li, H.

AU - Cai, H.

AU - Chen, S. M.

AU - Deng, G. R.

AU - Liu, P. N.

PY - 1986/7

Y1 - 1986/7

N2 - DNAs of three cell lines of human gastrocarcinoma (MGC-803, BGC-823 and PACM-82) and two fresh solid tumors of human stomach cancer were used to transfect NIH3T3 and Rat-1 cells. The transformed cells were selected with high concentration of glucose and low concentration of serum, or with medium containing Geneticin (G418) after co-transfection of pSVneo and DNAs of stomach cancer cell line or primary transformants. From the second round transfection, we had obtained transformants which could grow with high colony forming efficiency in soft agarose and were tumorigenic in nude mice. The southern blot analysis showed that the cellular DNA of the transformants contained human Alu repeat sequence and the transformed gene from stomach cancer cell line (BGC-823) and was homologous to proto-oncogene c-Ha-ras. The transforming gene is able to induce neoplastic transformation of NIH3T3 and Rat-1 cells.

AB - DNAs of three cell lines of human gastrocarcinoma (MGC-803, BGC-823 and PACM-82) and two fresh solid tumors of human stomach cancer were used to transfect NIH3T3 and Rat-1 cells. The transformed cells were selected with high concentration of glucose and low concentration of serum, or with medium containing Geneticin (G418) after co-transfection of pSVneo and DNAs of stomach cancer cell line or primary transformants. From the second round transfection, we had obtained transformants which could grow with high colony forming efficiency in soft agarose and were tumorigenic in nude mice. The southern blot analysis showed that the cellular DNA of the transformants contained human Alu repeat sequence and the transformed gene from stomach cancer cell line (BGC-823) and was homologous to proto-oncogene c-Ha-ras. The transforming gene is able to induce neoplastic transformation of NIH3T3 and Rat-1 cells.

UR - https://www.scopus.com/pages/publications/0022742550

M3 - Article

C2 - 3757734

AN - SCOPUS:0022742550

SN - 0253-3766

VL - 8

SP - 241

EP - 244

JO - Chinese Journal of Oncology

JF - Chinese Journal of Oncology

IS - 4

ER -

DNA transformation activity of a human gastrocarcinoma cell line

Abstract

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