DNA Photolyase Repairs the trans-syn Cyclobutane Thymine Dimer

Sang Tae Kim; Khushbeer Malhotra; Aziz Sancar; Colin A. Smith; John Stephen Taylor

doi:10.1021/bi00079a001

DNA Photolyase Repairs the trans-syn Cyclobutane Thymine Dimer

Sang Tae Kim
, Khushbeer Malhotra
, Aziz Sancar
, Colin A. Smith
, John Stephen Taylor

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

DNA photolyases catalyze the splitting of the cyclobutane ring joining the two dihydropyrimidines of a pyrimidine dimer by a photoinduced electron-transfer reaction. Previous studies concluded that photolyase repairs only the cis-syn form of the eight stereoisomers of the cyclobutane pyrimidine dimer (Pyr[]Pyr). In this study we found that Escherichia coli photolyase binds to the trans-syn-I isomer of T[]T with about 10⁴-fold lower affinity than the cis-syn isomer but it repairs it relatively efficiently.

Original language	English
Pages (from-to)	7065-7068
Number of pages	4
Journal	Biochemistry
Volume	32
Issue number	28
DOIs	https://doi.org/10.1021/bi00079a001
State	Published - 1993

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title = "DNA Photolyase Repairs the trans-syn Cyclobutane Thymine Dimer",

abstract = "DNA photolyases catalyze the splitting of the cyclobutane ring joining the two dihydropyrimidines of a pyrimidine dimer by a photoinduced electron-transfer reaction. Previous studies concluded that photolyase repairs only the cis-syn form of the eight stereoisomers of the cyclobutane pyrimidine dimer (Pyr[]Pyr). In this study we found that Escherichia coli photolyase binds to the trans-syn-I isomer of T[]T with about 104-fold lower affinity than the cis-syn isomer but it repairs it relatively efficiently.",

author = "Kim, \{Sang Tae\} and Khushbeer Malhotra and Aziz Sancar and Smith, \{Colin A.\} and Taylor, \{John Stephen\}",

year = "1993",

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language = "English",

volume = "32",

pages = "7065--7068",

journal = "Biochemistry",

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T1 - DNA Photolyase Repairs the trans-syn Cyclobutane Thymine Dimer

AU - Kim, Sang Tae

AU - Malhotra, Khushbeer

AU - Sancar, Aziz

AU - Smith, Colin A.

AU - Taylor, John Stephen

PY - 1993

Y1 - 1993

N2 - DNA photolyases catalyze the splitting of the cyclobutane ring joining the two dihydropyrimidines of a pyrimidine dimer by a photoinduced electron-transfer reaction. Previous studies concluded that photolyase repairs only the cis-syn form of the eight stereoisomers of the cyclobutane pyrimidine dimer (Pyr[]Pyr). In this study we found that Escherichia coli photolyase binds to the trans-syn-I isomer of T[]T with about 104-fold lower affinity than the cis-syn isomer but it repairs it relatively efficiently.

AB - DNA photolyases catalyze the splitting of the cyclobutane ring joining the two dihydropyrimidines of a pyrimidine dimer by a photoinduced electron-transfer reaction. Previous studies concluded that photolyase repairs only the cis-syn form of the eight stereoisomers of the cyclobutane pyrimidine dimer (Pyr[]Pyr). In this study we found that Escherichia coli photolyase binds to the trans-syn-I isomer of T[]T with about 104-fold lower affinity than the cis-syn isomer but it repairs it relatively efficiently.

UR - https://www.scopus.com/pages/publications/0027282986

U2 - 10.1021/bi00079a001

DO - 10.1021/bi00079a001

M3 - Article

C2 - 8343500

AN - SCOPUS:0027282986

SN - 0006-2960

VL - 32

SP - 7065

EP - 7068

JO - Biochemistry

JF - Biochemistry

IS - 28

ER -

DNA Photolyase Repairs the trans-syn Cyclobutane Thymine Dimer

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