DNA Photolyase Repairs the trans-syn Cyclobutane Thymine Dimer

Sang Tae Kim; Khushbeer Malhotra; Aziz Sancar; Colin A. Smith; John Stephen Taylor

doi:10.1021/bi00079a001

DNA Photolyase Repairs the trans-syn Cyclobutane Thymine Dimer

Sang Tae Kim, Khushbeer Malhotra, Aziz Sancar, Colin A. Smith, John Stephen Taylor

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30 Scopus citations

Abstract

DNA photolyases catalyze the splitting of the cyclobutane ring joining the two dihydropyrimidines of a pyrimidine dimer by a photoinduced electron-transfer reaction. Previous studies concluded that photolyase repairs only the cis-syn form of the eight stereoisomers of the cyclobutane pyrimidine dimer (Pyr[]Pyr). In this study we found that Escherichia coli photolyase binds to the trans-syn-I isomer of T[]T with about 10⁴-fold lower affinity than the cis-syn isomer but it repairs it relatively efficiently.

Original language	English
Pages (from-to)	7065-7068
Number of pages	4
Journal	Biochemistry
Volume	32
Issue number	28
DOIs	https://doi.org/10.1021/bi00079a001
State	Published - 1993

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abstract = "DNA photolyases catalyze the splitting of the cyclobutane ring joining the two dihydropyrimidines of a pyrimidine dimer by a photoinduced electron-transfer reaction. Previous studies concluded that photolyase repairs only the cis-syn form of the eight stereoisomers of the cyclobutane pyrimidine dimer (Pyr[]Pyr). In this study we found that Escherichia coli photolyase binds to the trans-syn-I isomer of T[]T with about 104-fold lower affinity than the cis-syn isomer but it repairs it relatively efficiently.",

author = "Kim, {Sang Tae} and Khushbeer Malhotra and Aziz Sancar and Smith, {Colin A.} and Taylor, {John Stephen}",

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T1 - DNA Photolyase Repairs the trans-syn Cyclobutane Thymine Dimer

AU - Kim, Sang Tae

AU - Malhotra, Khushbeer

AU - Sancar, Aziz

AU - Smith, Colin A.

AU - Taylor, John Stephen

PY - 1993

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AB - DNA photolyases catalyze the splitting of the cyclobutane ring joining the two dihydropyrimidines of a pyrimidine dimer by a photoinduced electron-transfer reaction. Previous studies concluded that photolyase repairs only the cis-syn form of the eight stereoisomers of the cyclobutane pyrimidine dimer (Pyr[]Pyr). In this study we found that Escherichia coli photolyase binds to the trans-syn-I isomer of T[]T with about 104-fold lower affinity than the cis-syn isomer but it repairs it relatively efficiently.

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JO - Biochemistry

JF - Biochemistry

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ER -

DNA Photolyase Repairs the trans-syn Cyclobutane Thymine Dimer

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