DNA methylation in the TNF-alpha gene decreases along with aging among delirium inpatients

Takehiko Yamanashi; Taku Saito; Tong Yu; Alexandra Alario; Katie Comp; Kaitlyn J. Crutchley; Eleanor J. Sullivan; Zoe Ella M. Anderson; Pedro S. Marra; Gloria Chang; Nadia E. Wahba; Sydney S. Jellison; Alissa A. Meyer; Srishti Mathur; Pratik Pandharipande; Aihide Yoshino; Koichi Kaneko; Sangil Lee; Hiroyuki Toda; Masaaki Iwata; Gen Shinozaki

doi:10.1016/j.neurobiolaging.2021.05.005

DNA methylation in the TNF-alpha gene decreases along with aging among delirium inpatients

Takehiko Yamanashi, Taku Saito, Tong Yu, Alexandra Alario, Katie Comp, Kaitlyn J. Crutchley, Eleanor J. Sullivan, Zoe Ella M. Anderson, Pedro S. Marra, Gloria Chang, Nadia E. Wahba, Sydney S. Jellison, Alissa A. Meyer, Srishti Mathur, Pratik Pandharipande, Aihide Yoshino, Koichi Kaneko, Sangil Lee, Hiroyuki Toda, Masaaki IwataGen Shinozaki

Division of Hospital Medicine

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

It has been suggested that aging and inflammation play key roles in the development of delirium. In the present study, we investigated the differences of the DNAm patterns in the TNF gene between patients with delirium and without. The data and samples derived from previous and ongoing cohort studies were analyzed. DNAm levels of the TNF gene were analyzed using the Illumina EPIC array genome-wide method and pyrosequencing method. Correlations between age and DNAm levels of each CpG were calculated. Several CpG in the TNF gene in blood showed negative correlation between their DNAm and age in delirium cases both with the EPIC array and by the pyrosequencing method. However, there was no CpG that had significant correlation between their DNAm and age regardless of delirium status among buccal samples. On the other hand, among peripheral blood mononuclear cells samples, it was found that several CpG showed negative correlation between their DNAm and age in delirium cases. The evidence of DNAm change in the TNF gene among delirious subjects was demonstrated.

Original language	English
Pages (from-to)	310-317
Number of pages	8
Journal	Neurobiology of Aging
Volume	105
DOIs	https://doi.org/10.1016/j.neurobiolaging.2021.05.005
State	Published - Sep 2021

Keywords

Aging
Delirium
DNA methylation
EPIC array
Pyrosequencing
TNF-alpha

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10.1016/j.neurobiolaging.2021.05.005

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Yamanashi, T., Saito, T., Yu, T., Alario, A., Comp, K., Crutchley, K. J., Sullivan, E. J., Anderson, Z. E. M., Marra, P. S., Chang, G., Wahba, N. E., Jellison, S. S., Meyer, A. A., Mathur, S., Pandharipande, P., Yoshino, A., Kaneko, K., Lee, S., Toda, H., ... Shinozaki, G. (2021). DNA methylation in the TNF-alpha gene decreases along with aging among delirium inpatients. Neurobiology of Aging, 105, 310-317. https://doi.org/10.1016/j.neurobiolaging.2021.05.005

@article{1edbbd42339a44dcb20f2e7bae676d27,

title = "DNA methylation in the TNF-alpha gene decreases along with aging among delirium inpatients",

abstract = "It has been suggested that aging and inflammation play key roles in the development of delirium. In the present study, we investigated the differences of the DNAm patterns in the TNF gene between patients with delirium and without. The data and samples derived from previous and ongoing cohort studies were analyzed. DNAm levels of the TNF gene were analyzed using the Illumina EPIC array genome-wide method and pyrosequencing method. Correlations between age and DNAm levels of each CpG were calculated. Several CpG in the TNF gene in blood showed negative correlation between their DNAm and age in delirium cases both with the EPIC array and by the pyrosequencing method. However, there was no CpG that had significant correlation between their DNAm and age regardless of delirium status among buccal samples. On the other hand, among peripheral blood mononuclear cells samples, it was found that several CpG showed negative correlation between their DNAm and age in delirium cases. The evidence of DNAm change in the TNF gene among delirious subjects was demonstrated.",

keywords = "Aging, Delirium, DNA methylation, EPIC array, Pyrosequencing, TNF-alpha",

author = "Takehiko Yamanashi and Taku Saito and Tong Yu and Alexandra Alario and Katie Comp and Crutchley, {Kaitlyn J.} and Sullivan, {Eleanor J.} and Anderson, {Zoe Ella M.} and Marra, {Pedro S.} and Gloria Chang and Wahba, {Nadia E.} and Jellison, {Sydney S.} and Meyer, {Alissa A.} and Srishti Mathur and Pratik Pandharipande and Aihide Yoshino and Koichi Kaneko and Sangil Lee and Hiroyuki Toda and Masaaki Iwata and Gen Shinozaki",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",

year = "2021",

month = sep,

doi = "10.1016/j.neurobiolaging.2021.05.005",

language = "English",

volume = "105",

pages = "310--317",

journal = "Neurobiology of Aging",

issn = "0197-4580",

}

Yamanashi, T, Saito, T, Yu, T, Alario, A, Comp, K, Crutchley, KJ, Sullivan, EJ, Anderson, ZEM, Marra, PS, Chang, G, Wahba, NE, Jellison, SS, Meyer, AA, Mathur, S, Pandharipande, P, Yoshino, A, Kaneko, K, Lee, S, Toda, H, Iwata, M & Shinozaki, G 2021, 'DNA methylation in the TNF-alpha gene decreases along with aging among delirium inpatients', Neurobiology of Aging, vol. 105, pp. 310-317. https://doi.org/10.1016/j.neurobiolaging.2021.05.005

TY - JOUR

T1 - DNA methylation in the TNF-alpha gene decreases along with aging among delirium inpatients

AU - Yamanashi, Takehiko

AU - Saito, Taku

AU - Yu, Tong

AU - Alario, Alexandra

AU - Comp, Katie

AU - Crutchley, Kaitlyn J.

AU - Sullivan, Eleanor J.

AU - Anderson, Zoe Ella M.

AU - Marra, Pedro S.

AU - Chang, Gloria

AU - Wahba, Nadia E.

AU - Jellison, Sydney S.

AU - Meyer, Alissa A.

AU - Mathur, Srishti

AU - Pandharipande, Pratik

AU - Yoshino, Aihide

AU - Kaneko, Koichi

AU - Lee, Sangil

AU - Toda, Hiroyuki

AU - Iwata, Masaaki

AU - Shinozaki, Gen

PY - 2021/9

Y1 - 2021/9

N2 - It has been suggested that aging and inflammation play key roles in the development of delirium. In the present study, we investigated the differences of the DNAm patterns in the TNF gene between patients with delirium and without. The data and samples derived from previous and ongoing cohort studies were analyzed. DNAm levels of the TNF gene were analyzed using the Illumina EPIC array genome-wide method and pyrosequencing method. Correlations between age and DNAm levels of each CpG were calculated. Several CpG in the TNF gene in blood showed negative correlation between their DNAm and age in delirium cases both with the EPIC array and by the pyrosequencing method. However, there was no CpG that had significant correlation between their DNAm and age regardless of delirium status among buccal samples. On the other hand, among peripheral blood mononuclear cells samples, it was found that several CpG showed negative correlation between their DNAm and age in delirium cases. The evidence of DNAm change in the TNF gene among delirious subjects was demonstrated.

AB - It has been suggested that aging and inflammation play key roles in the development of delirium. In the present study, we investigated the differences of the DNAm patterns in the TNF gene between patients with delirium and without. The data and samples derived from previous and ongoing cohort studies were analyzed. DNAm levels of the TNF gene were analyzed using the Illumina EPIC array genome-wide method and pyrosequencing method. Correlations between age and DNAm levels of each CpG were calculated. Several CpG in the TNF gene in blood showed negative correlation between their DNAm and age in delirium cases both with the EPIC array and by the pyrosequencing method. However, there was no CpG that had significant correlation between their DNAm and age regardless of delirium status among buccal samples. On the other hand, among peripheral blood mononuclear cells samples, it was found that several CpG showed negative correlation between their DNAm and age in delirium cases. The evidence of DNAm change in the TNF gene among delirious subjects was demonstrated.

KW - Aging

KW - Delirium

KW - DNA methylation

KW - EPIC array

KW - Pyrosequencing

KW - TNF-alpha

UR - http://www.scopus.com/inward/record.url?scp=85110494996&partnerID=8YFLogxK

U2 - 10.1016/j.neurobiolaging.2021.05.005

DO - 10.1016/j.neurobiolaging.2021.05.005

M3 - Article

C2 - 34192631

AN - SCOPUS:85110494996

SN - 0197-4580

VL - 105

SP - 310

EP - 317

JO - Neurobiology of Aging

JF - Neurobiology of Aging

ER -

DNA methylation in the TNF-alpha gene decreases along with aging among delirium inpatients

Abstract

Keywords

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