DNA Methylation and Regulatory Elements during Chicken Germline Stem Cell Differentiation

Yanghua He, Qisheng Zuo, John Edwards, Keji Zhao, Jinzhi Lei, Wentao Cai, Qing Nie, Bichun Li, Jiuzhou Song

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The production of germ cells in vitro would open important new avenues for stem biology and human medicine, but the mechanisms of germ cell differentiation are not well understood. The chicken, as a great model for embryology and development, was used in this study to help us explore its regulatory mechanisms. In this study, we reported a comprehensive genome-wide DNA methylation landscape in chicken germ cells, and transcriptomic dynamics was also presented. By uncovering DNA methylation patterns on individual genes, some genes accurately modulated by DNA methylation were found to be associated with cancers and virus infection, e.g., AKT1 and CTNNB1. Chicken-unique markers were also discovered for identifying male germ cells. Importantly, integrated epigenetic mechanisms were explored during male germ cell differentiation, which provides deep insight into the epigenetic processes associated with male germ cell differentiation and possibly improves treatment options to male infertility in animals and humans. In this article, Song, Li, and their colleagues reported a comprehensive genome-wide DNA methylation landscape in chicken germ cells. The integrated epigenetic mechanisms were explored, specifically, germ cell differentiation experiences through a highly orchestrated process that involves multiple epigenetic events, which would give a clue for curing male infertility in animals and humans.

Original languageEnglish
Pages (from-to)1793-1806
Number of pages14
JournalStem Cell Reports
Volume10
Issue number6
DOIs
StatePublished - Jun 5 2018

Keywords

  • DNA methylation
  • ESCs
  • PGCs
  • SSCs
  • chicken
  • epigenetics
  • gene expression
  • germline stem cell differentiation
  • non-coding RNAs
  • transcription factor motifs

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