DNA methylation and regulation of the human β-globin-like genes in mouse erythroleukemia cells containing human chromosome 11

T. J. Ley; Y. L. Chiang; D. Haidaris; N. P. Anagnou; V. L. Wilson; W. F. Anderson

doi:10.1073/pnas.81.21.6618

DNA methylation and regulation of the human β-globin-like genes in mouse erythroleukemia cells containing human chromosome 11

T. J. Ley
, Y. L. Chiang
, D. Haidaris
, N. P. Anagnou
, V. L. Wilson
, W. F. Anderson

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The human β-globin gene is expressed - but the human fetal (γ) and embryonic (ε) globin genes are not - in an induced mouse erythroleukemia cell line (M11-X) that contains most of human chromosome 11. A 24-hr exposure of M11-X cells to 5-azacytidine before induction causes 'global' DNA hypomethylation but selective activation of the human γ-globin genes. Genomic DNA is remethylated 2-3 days after exposure to 5-azacytidine, but sequences near the human and mouse globin genes remain hypomethylated, suggesting that the remethylation process is inhibited in these regions.

Original language	English
Pages (from-to)	6618-6622
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	81
Issue number	21 I
DOIs	https://doi.org/10.1073/pnas.81.21.6618
State	Published - 1984

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title = "DNA methylation and regulation of the human β-globin-like genes in mouse erythroleukemia cells containing human chromosome 11",

abstract = "The human β-globin gene is expressed - but the human fetal (γ) and embryonic (ε) globin genes are not - in an induced mouse erythroleukemia cell line (M11-X) that contains most of human chromosome 11. A 24-hr exposure of M11-X cells to 5-azacytidine before induction causes 'global' DNA hypomethylation but selective activation of the human γ-globin genes. Genomic DNA is remethylated 2-3 days after exposure to 5-azacytidine, but sequences near the human and mouse globin genes remain hypomethylated, suggesting that the remethylation process is inhibited in these regions.",

author = "Ley, \{T. J.\} and Chiang, \{Y. L.\} and D. Haidaris and Anagnou, \{N. P.\} and Wilson, \{V. L.\} and Anderson, \{W. F.\}",

year = "1984",

doi = "10.1073/pnas.81.21.6618",

language = "English",

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journal = "Proceedings of the National Academy of Sciences of the United States of America",

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T1 - DNA methylation and regulation of the human β-globin-like genes in mouse erythroleukemia cells containing human chromosome 11

AU - Ley, T. J.

AU - Chiang, Y. L.

AU - Haidaris, D.

AU - Anagnou, N. P.

AU - Wilson, V. L.

AU - Anderson, W. F.

PY - 1984

Y1 - 1984

N2 - The human β-globin gene is expressed - but the human fetal (γ) and embryonic (ε) globin genes are not - in an induced mouse erythroleukemia cell line (M11-X) that contains most of human chromosome 11. A 24-hr exposure of M11-X cells to 5-azacytidine before induction causes 'global' DNA hypomethylation but selective activation of the human γ-globin genes. Genomic DNA is remethylated 2-3 days after exposure to 5-azacytidine, but sequences near the human and mouse globin genes remain hypomethylated, suggesting that the remethylation process is inhibited in these regions.

AB - The human β-globin gene is expressed - but the human fetal (γ) and embryonic (ε) globin genes are not - in an induced mouse erythroleukemia cell line (M11-X) that contains most of human chromosome 11. A 24-hr exposure of M11-X cells to 5-azacytidine before induction causes 'global' DNA hypomethylation but selective activation of the human γ-globin genes. Genomic DNA is remethylated 2-3 days after exposure to 5-azacytidine, but sequences near the human and mouse globin genes remain hypomethylated, suggesting that the remethylation process is inhibited in these regions.

UR - https://www.scopus.com/pages/publications/0021703469

U2 - 10.1073/pnas.81.21.6618

DO - 10.1073/pnas.81.21.6618

M3 - Article

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AN - SCOPUS:0021703469

SN - 0027-8424

VL - 81

SP - 6618

EP - 6622

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 21 I

ER -

DNA methylation and regulation of the human β-globin-like genes in mouse erythroleukemia cells containing human chromosome 11

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