DNA methylation and regulation of the human β-globin-like genes in mouse erythroleukemia cells containing human chromosome 11

T. J. Ley, Y. L. Chiang, D. Haidaris, N. P. Anagnou, V. L. Wilson, W. F. Anderson

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The human β-globin gene is expressed - but the human fetal (γ) and embryonic (ε) globin genes are not - in an induced mouse erythroleukemia cell line (M11-X) that contains most of human chromosome 11. A 24-hr exposure of M11-X cells to 5-azacytidine before induction causes 'global' DNA hypomethylation but selective activation of the human γ-globin genes. Genomic DNA is remethylated 2-3 days after exposure to 5-azacytidine, but sequences near the human and mouse globin genes remain hypomethylated, suggesting that the remethylation process is inhibited in these regions.

Original languageEnglish
Pages (from-to)6618-6622
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number21 I
DOIs
StatePublished - 1984

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