DNA "fingerprints" applied to paternity analysis in apples (Malus x domestica)

H. Nybom; B. A. Schaal

doi:10.1007/BF00224242

DNA "fingerprints" applied to paternity analysis in apples (Malus x domestica)

H. Nybom, B. A. Schaal

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Analysis of minisatellite DNA sequences, yielding so called DNA "fingerprints", has proven useful in paternity analysis for several different organisms. Here 64 apple seedlings, grown from seeds collected in an orchard with three cultivars, were analyzed using the M13 "fingerprint" probe. Paternity could be determined for 56 of the seedlings, 2 of which were derived through selfing. The analysis was facilitated by the occurrence of a multiallelic locus. The five different fragments determined by this locus migrated to similar positions, whether digesting the DNA with restriction enzymes TaqI or RsaI.

Original language	English
Pages (from-to)	763-768
Number of pages	6
Journal	Theoretical and Applied Genetics
Volume	79
Issue number	6
DOIs	https://doi.org/10.1007/BF00224242
State	Published - Jun 1990

Keywords

Apple
DNA fingerprint
M13 probe
Paternity analysis
RFLP

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10.1007/BF00224242

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abstract = "Analysis of minisatellite DNA sequences, yielding so called DNA {"}fingerprints{"}, has proven useful in paternity analysis for several different organisms. Here 64 apple seedlings, grown from seeds collected in an orchard with three cultivars, were analyzed using the M13 {"}fingerprint{"} probe. Paternity could be determined for 56 of the seedlings, 2 of which were derived through selfing. The analysis was facilitated by the occurrence of a multiallelic locus. The five different fragments determined by this locus migrated to similar positions, whether digesting the DNA with restriction enzymes TaqI or RsaI.",

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AU - Nybom, H.

AU - Schaal, B. A.

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N2 - Analysis of minisatellite DNA sequences, yielding so called DNA "fingerprints", has proven useful in paternity analysis for several different organisms. Here 64 apple seedlings, grown from seeds collected in an orchard with three cultivars, were analyzed using the M13 "fingerprint" probe. Paternity could be determined for 56 of the seedlings, 2 of which were derived through selfing. The analysis was facilitated by the occurrence of a multiallelic locus. The five different fragments determined by this locus migrated to similar positions, whether digesting the DNA with restriction enzymes TaqI or RsaI.

AB - Analysis of minisatellite DNA sequences, yielding so called DNA "fingerprints", has proven useful in paternity analysis for several different organisms. Here 64 apple seedlings, grown from seeds collected in an orchard with three cultivars, were analyzed using the M13 "fingerprint" probe. Paternity could be determined for 56 of the seedlings, 2 of which were derived through selfing. The analysis was facilitated by the occurrence of a multiallelic locus. The five different fragments determined by this locus migrated to similar positions, whether digesting the DNA with restriction enzymes TaqI or RsaI.

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KW - DNA fingerprint

KW - M13 probe

KW - Paternity analysis

KW - RFLP

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IS - 6

ER -

DNA "fingerprints" applied to paternity analysis in apples (Malus x domestica)

Abstract

Keywords

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