TY - JOUR
T1 - DNA double-strand breaks induce H2Ax phosphorylation domains in a contact-dependent manner
AU - Collins, Patrick L.
AU - Purman, Caitlin
AU - Porter, Sofia I.
AU - Nganga, Vincent
AU - Saini, Ankita
AU - Hayer, Katharina E.
AU - Gurewitz, Greer L.
AU - Sleckman, Barry P.
AU - Bednarski, Jeffrey J.
AU - Bassing, Craig H.
AU - Oltz, Eugene M.
N1 - Funding Information:
The Genome Technology Access Center is partially supported by National Cancer Institute Cancer Center Support Grant P30 CA91842 (to the Siteman Cancer Center) and by Institute of Clinical and Translational Sciences (ICTS/CTSA) GrantUL1TR000448 from the National Center for Research Resources. This work was supported by National Institutes of Health (NIH) RO1 AI130231 (E.M.O.) and AI118852 (E.M.O. and C.B.) K08 AI102946 (J.J.B.), Alex’s Lemonade Stand Foundation (J.J.B.), the Foundation for Barnes-Jewish Hospital Cancer Frontier Fund (J.J.B.), the Barnard Trust (J.J.B.), and an American Society of Hematology Scholar Award (J.J.B.).
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Efficient repair of DNA double-strand breaks (DSBs) requires a coordinated DNA Damage Response (DDR), which includes phosphorylation of histone H2Ax, forming γH2Ax. This histone modification spreads beyond the DSB into neighboring chromatin, generating a DDR platform that protects against end disassociation and degradation, minimizing chromosomal rearrangements. However, mechanisms that determine the breadth and intensity of γH2Ax domains remain unclear. Here, we show that chromosomal contacts of a DSB site are the primary determinants for γH2Ax landscapes. DSBs that disrupt a topological border permit extension of γH2Ax domains into both adjacent compartments. In contrast, DSBs near a border produce highly asymmetric DDR platforms, with γH2Ax nearly absent from one broken end. Collectively, our findings lend insights into a basic DNA repair mechanism and how the precise location of a DSB may influence genome integrity.
AB - Efficient repair of DNA double-strand breaks (DSBs) requires a coordinated DNA Damage Response (DDR), which includes phosphorylation of histone H2Ax, forming γH2Ax. This histone modification spreads beyond the DSB into neighboring chromatin, generating a DDR platform that protects against end disassociation and degradation, minimizing chromosomal rearrangements. However, mechanisms that determine the breadth and intensity of γH2Ax domains remain unclear. Here, we show that chromosomal contacts of a DSB site are the primary determinants for γH2Ax landscapes. DSBs that disrupt a topological border permit extension of γH2Ax domains into both adjacent compartments. In contrast, DSBs near a border produce highly asymmetric DDR platforms, with γH2Ax nearly absent from one broken end. Collectively, our findings lend insights into a basic DNA repair mechanism and how the precise location of a DSB may influence genome integrity.
UR - http://www.scopus.com/inward/record.url?scp=85086788644&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-16926-x
DO - 10.1038/s41467-020-16926-x
M3 - Article
C2 - 32572033
AN - SCOPUS:85086788644
VL - 11
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 3158
ER -