TY - JOUR
T1 - DL-buthionine-S,R-sulfoximine affects intestinal alkaline phosphatase activity
AU - Marchionatti, Ana
AU - Alisio, Arturo
AU - Díaz De Barboza, Gabriela
AU - Baudino, Vanessa
AU - Tolosa De Talamoni, Nori
N1 - Funding Information:
This study was supported by grants from FONCYT (BID 802/OC-AR PICT 98-05-03126) and SECYT (UNC), Argentina. Dr Nori Tolosa de Talamoni is a member of the Investigator Career from the CONICET, Gabriela Dı́az de Barboza is a recipient of a Fellowship from SECYT (UNC).
PY - 2001
Y1 - 2001
N2 - The susceptibility of intestinal alkaline phosphatase to DL-buthionine-S,R-sulfoximine was investigated in chicks fed a commercial diet. The results show that DL-buthionine-S,R-sulfoximine produced inhibition of intestinal alkaline phosphatase activity. This effect showed dose- and time-dependency and it was caused by either in vivo DL-buthionine-S,R- sulfoximine administration or in vitro DL-buthionine-S,R-sulfoximine incubation with villus tip enterocytes. DL-Buthionine-S,R-sulfoximine did not act directly on intestinal alkaline phosphatase but it provoked glutathione depletion which led to changes in the redox state of the enterocyte as shown by the production of free hydroxyl radicals and an incremental increase in the carbonyl content of proteins. The reversibility of the buthionine sulfoximine effect on intestinal alkaline phosphatase was proved by addition of glutathione monoester to the duodenal loop.
AB - The susceptibility of intestinal alkaline phosphatase to DL-buthionine-S,R-sulfoximine was investigated in chicks fed a commercial diet. The results show that DL-buthionine-S,R-sulfoximine produced inhibition of intestinal alkaline phosphatase activity. This effect showed dose- and time-dependency and it was caused by either in vivo DL-buthionine-S,R- sulfoximine administration or in vitro DL-buthionine-S,R-sulfoximine incubation with villus tip enterocytes. DL-Buthionine-S,R-sulfoximine did not act directly on intestinal alkaline phosphatase but it provoked glutathione depletion which led to changes in the redox state of the enterocyte as shown by the production of free hydroxyl radicals and an incremental increase in the carbonyl content of proteins. The reversibility of the buthionine sulfoximine effect on intestinal alkaline phosphatase was proved by addition of glutathione monoester to the duodenal loop.
KW - Alkaline phosphatase
KW - Chicks
KW - DL-buthionine-S,R-sulfoximine
KW - Free hydroxyl radicals
KW - Glutathione
KW - Glutathione monoester
KW - Intestine
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=0034968438&partnerID=8YFLogxK
U2 - 10.1016/S1532-0456(01)00185-5
DO - 10.1016/S1532-0456(01)00185-5
M3 - Article
C2 - 11423381
AN - SCOPUS:0034968438
SN - 1532-0456
VL - 129
SP - 85
EP - 91
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
IS - 2
ER -