TY - JOUR
T1 - Diverse suppressors of RNA silencing enhance agroinfection by a viral replicon
AU - Chiba, Marin
AU - Reed, Jonathan C.
AU - Prokhnevsky, Alexey I.
AU - Chapman, Elisabeth J.
AU - Mawassi, Munir
AU - Koonin, Eugene V.
AU - Carrington, James C.
AU - Dolja, Valerian V.
N1 - Funding Information:
We thank Shou-Wei Ding for stimulating discussions. The cDNA clones used in this work were kindly provided by Nina Abou-Ghanem, David Baulcombe, Shou-Wei Ding, Jack Morris, Herman Scholthof, and Vicki Vance. This work was supported by grants from the National Institutes of Health (GM053190), US Department of Agriculture (NRI 2001-35319-10875), and Large Scale Biology Corp. (2000-13) to V.V.D., the National Science Foundation (MCB-0209836) and the National Institutes of Health (AI43288) to J.C.C., and BARD (IS-3784-05) to M.M. and V.V.D. This publication was made possible in part by grant number 1S10RR107903-01 from the National Institutes of Health. The authors wish to acknowledge the Confocal Microscopy Facility of the Center for Gene Research and Biotechnology and the Environmental and Health Sciences Center at Oregon State University.
PY - 2006/3/1
Y1 - 2006/3/1
N2 - Launching the Beet yellows virus (BYV) minireplicon by agrobacterial delivery resulted in an unexpectedly low number of infected cells per inoculated leaf. This effect was due to a strong RNA silencing response in the agroinfiltrated leaves. Strikingly, ectopic co-expression of p21, a BYV RNA silencing suppressor, increased minireplicon infectivity by three orders of magnitude. Mutational analysis demonstrated that this effect correlates with suppressor activity of p21. Five diverse, heterologous viral suppressors were also active in this system, providing a useful approach for a dramatic, up to 10,000-fold, increase of the efficiency of agroinfection. The minireplicon agroinfection assay was also used to identify a new suppressor, a homolog of BYV p21, derived from Grapevine leafroll-associated virus-2. In addition, we report preliminary data on the suppressor activity of the p10 protein of Grapevine virus A and show that this protein belongs to a family of Zn-ribbon-containing proteins encoded by filamentous plant RNA viruses from three genera. The members of this family are predicted to have RNA silencing suppressor activity.
AB - Launching the Beet yellows virus (BYV) minireplicon by agrobacterial delivery resulted in an unexpectedly low number of infected cells per inoculated leaf. This effect was due to a strong RNA silencing response in the agroinfiltrated leaves. Strikingly, ectopic co-expression of p21, a BYV RNA silencing suppressor, increased minireplicon infectivity by three orders of magnitude. Mutational analysis demonstrated that this effect correlates with suppressor activity of p21. Five diverse, heterologous viral suppressors were also active in this system, providing a useful approach for a dramatic, up to 10,000-fold, increase of the efficiency of agroinfection. The minireplicon agroinfection assay was also used to identify a new suppressor, a homolog of BYV p21, derived from Grapevine leafroll-associated virus-2. In addition, we report preliminary data on the suppressor activity of the p10 protein of Grapevine virus A and show that this protein belongs to a family of Zn-ribbon-containing proteins encoded by filamentous plant RNA viruses from three genera. The members of this family are predicted to have RNA silencing suppressor activity.
KW - Agroinfection
KW - RNA silencing
KW - Viral suppressors
UR - https://www.scopus.com/pages/publications/32844469710
U2 - 10.1016/j.virol.2005.09.068
DO - 10.1016/j.virol.2005.09.068
M3 - Article
C2 - 16300814
AN - SCOPUS:32844469710
SN - 0042-6822
VL - 346
SP - 7
EP - 14
JO - Virology
JF - Virology
IS - 1
ER -