OBJECTIVE: Our objective was to study the combined effect of taxol and tumor necrosis factor-a on the cytolysis of human ovarian carcinoma cell lines, because taxol has been shown to be active against ovarian carcinoma and has also been shown to increase tumor necrosis, factor-α. release from macrophages. STUDY DESIGN: The combined effect of taxol and tumor necrosis factor-α. on the cell lines Caov-3, SK-OV-3, NIH:OVCAR-3, and A2780, which are sensitive to the cytolytic effect of tumor necrosis factor-α in the presence of inhibitors of protein synthesis, was investigated with a 24-hour chromium 51 release assay. RESULTS: At therapeutic concentrations taxol caused a significant increase in tumor necrosis factor-α-mediated cytolysis of Caov-3 and A2780 (p ≤ 0.05). By contrast; taxol caused a decrease in the tumor necrosis factor-α-mediated cytolysis of SK-OV-3 and NIH:OVCAR-3 (p ≤ 0.01). CONCLUSION: These results suggest that ovarian carcinomas have a heterogeneous response to the chemotherapeutic effect of taxol.
- ovarian carcinoma
- tumor necrosis factor-a