TY - JOUR
T1 - Divergent effects of taxol on tumor necrosis factor-α-mediated cytolysis of ovarian carcinoma cells
AU - Williams, Sybilann
AU - Mutch, David G.
AU - Xu, Ling
AU - Collins, John Leslie
PY - 1992/12
Y1 - 1992/12
N2 - OBJECTIVE: Our objective was to study the combined effect of taxol and tumor necrosis factor-a on the cytolysis of human ovarian carcinoma cell lines, because taxol has been shown to be active against ovarian carcinoma and has also been shown to increase tumor necrosis, factor-α. release from macrophages. STUDY DESIGN: The combined effect of taxol and tumor necrosis factor-α. on the cell lines Caov-3, SK-OV-3, NIH:OVCAR-3, and A2780, which are sensitive to the cytolytic effect of tumor necrosis factor-α in the presence of inhibitors of protein synthesis, was investigated with a 24-hour chromium 51 release assay. RESULTS: At therapeutic concentrations taxol caused a significant increase in tumor necrosis factor-α-mediated cytolysis of Caov-3 and A2780 (p ≤ 0.05). By contrast; taxol caused a decrease in the tumor necrosis factor-α-mediated cytolysis of SK-OV-3 and NIH:OVCAR-3 (p ≤ 0.01). CONCLUSION: These results suggest that ovarian carcinomas have a heterogeneous response to the chemotherapeutic effect of taxol.
AB - OBJECTIVE: Our objective was to study the combined effect of taxol and tumor necrosis factor-a on the cytolysis of human ovarian carcinoma cell lines, because taxol has been shown to be active against ovarian carcinoma and has also been shown to increase tumor necrosis, factor-α. release from macrophages. STUDY DESIGN: The combined effect of taxol and tumor necrosis factor-α. on the cell lines Caov-3, SK-OV-3, NIH:OVCAR-3, and A2780, which are sensitive to the cytolytic effect of tumor necrosis factor-α in the presence of inhibitors of protein synthesis, was investigated with a 24-hour chromium 51 release assay. RESULTS: At therapeutic concentrations taxol caused a significant increase in tumor necrosis factor-α-mediated cytolysis of Caov-3 and A2780 (p ≤ 0.05). By contrast; taxol caused a decrease in the tumor necrosis factor-α-mediated cytolysis of SK-OV-3 and NIH:OVCAR-3 (p ≤ 0.01). CONCLUSION: These results suggest that ovarian carcinomas have a heterogeneous response to the chemotherapeutic effect of taxol.
KW - Taxol
KW - cytolysis
KW - ovarian carcinoma
KW - tumor necrosis factor-a
UR - http://www.scopus.com/inward/record.url?scp=0026740055&partnerID=8YFLogxK
U2 - 10.1016/0002-9378(92)91789-D
DO - 10.1016/0002-9378(92)91789-D
M3 - Article
C2 - 1361719
AN - SCOPUS:0026740055
SN - 0002-9378
VL - 167
SP - 1870
EP - 1876
JO - American journal of obstetrics and gynecology
JF - American journal of obstetrics and gynecology
IS - 6
ER -