Distribution of anionic sites in gut tissue: an electron-microscopical study

K. Dikranian; M. Petrov; K. Georgieva; G. Burnstock

doi:10.1007/BF00313979

Distribution of anionic sites in gut tissue: an electron-microscopical study

K. Dikranian, M. Petrov, K. Georgieva, G. Burnstock

Department of Neuroscience

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A new one-step incubation method using cationic gold colloid was applied to reveal anionic moieties in rat colonic mucosa. Gold particles were detected in all cellular nuclei, basement membranes, mast cell granules and collagen fibres, while the luminal surfaces of all vascular endothelial cells were devoid of gold label. Application of the method for detection of anionic domains under various conditions is discussed.

Original language	English
Pages (from-to)	385-387
Number of pages	3
Journal	Cell and Tissue Research
Volume	264
Issue number	2
DOIs	https://doi.org/10.1007/BF00313979
State	Published - May 1991

Keywords

Anionic sites
Cationic gold colloid
Rat (Wistar)

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10.1007/BF00313979

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title = "Distribution of anionic sites in gut tissue: an electron-microscopical study",

abstract = "A new one-step incubation method using cationic gold colloid was applied to reveal anionic moieties in rat colonic mucosa. Gold particles were detected in all cellular nuclei, basement membranes, mast cell granules and collagen fibres, while the luminal surfaces of all vascular endothelial cells were devoid of gold label. Application of the method for detection of anionic domains under various conditions is discussed.",

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AU - Dikranian, K.

AU - Petrov, M.

AU - Georgieva, K.

AU - Burnstock, G.

PY - 1991/5

Y1 - 1991/5

N2 - A new one-step incubation method using cationic gold colloid was applied to reveal anionic moieties in rat colonic mucosa. Gold particles were detected in all cellular nuclei, basement membranes, mast cell granules and collagen fibres, while the luminal surfaces of all vascular endothelial cells were devoid of gold label. Application of the method for detection of anionic domains under various conditions is discussed.

AB - A new one-step incubation method using cationic gold colloid was applied to reveal anionic moieties in rat colonic mucosa. Gold particles were detected in all cellular nuclei, basement membranes, mast cell granules and collagen fibres, while the luminal surfaces of all vascular endothelial cells were devoid of gold label. Application of the method for detection of anionic domains under various conditions is discussed.

KW - Anionic sites

KW - Cationic gold colloid

KW - Rat (Wistar)

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EP - 387

JO - Cell and Tissue Research

JF - Cell and Tissue Research

IS - 2

ER -

Distribution of anionic sites in gut tissue: an electron-microscopical study

Abstract

Keywords

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