TY - JOUR
T1 - Distribution of a macrophage-specific antigen
AU - Feldman, Joseph D.
AU - Tubergen, David G.
AU - Pollock, Elisabeth M.
AU - Unanue, Emil R.
N1 - Funding Information:
1 This work (publication No. 581) was supported by a United States Public Health Service (kant ~11-07007 and Atomic Energy Commission Contract AT(04-3)-779. ? Reprint requests are to be sent to: Joseph D. Feldman, M. D., Department of Experimental Pathology, Scripps Clinic and Research Foundation, 476 Prospect Street, La Jolla, C-4. 92037. 3 Supported by National Institutes of Health Grant 5TOl HD0057-10. l’resent address : Department of Pediatrics, University of Michigan Medical School, rZnn r\rbor, MI. 4P104. 4 Supported by United States Public Health Service Grant 5TIGM683-09. ’ Supported by Senior Fellowship of the -4merican Cancer Society, California Division. Resent address: Department of Pathology, Harvard Medical School. Boston, MA. 02115.
PY - 1972/10
Y1 - 1972/10
N2 - Macrophage specific antigens were detected with fluorescein and 125I-labeled rabbit antibodies to anti-guinea pig macrophages on surface membranes of 85-100% of peritoneal exudate macrophages (PEM), 35-90% of alveolar macrophages (AVM), 35-50% of splenic macrophages, and 10% of peripheral blood monocytes. None was detected in bone marrow cells, the source of precursor macrophages. Phagocytosis in vitro was not accompanied by an increase of labeled cells in bone marrow, blood, or spleen. Neither trypsin nor neuraminidase reduced macrophage-specific antigens on surface membranes of PEM nor uncovered such antigens in greater quantity in mononuclear phagocytes of bone marrow, blood or spleen. Neuraminidase augmented the binding of 125I-labeled antimacrophage globulins (AMG) and normal rabbit globulins to monocytes/macrophages.
AB - Macrophage specific antigens were detected with fluorescein and 125I-labeled rabbit antibodies to anti-guinea pig macrophages on surface membranes of 85-100% of peritoneal exudate macrophages (PEM), 35-90% of alveolar macrophages (AVM), 35-50% of splenic macrophages, and 10% of peripheral blood monocytes. None was detected in bone marrow cells, the source of precursor macrophages. Phagocytosis in vitro was not accompanied by an increase of labeled cells in bone marrow, blood, or spleen. Neither trypsin nor neuraminidase reduced macrophage-specific antigens on surface membranes of PEM nor uncovered such antigens in greater quantity in mononuclear phagocytes of bone marrow, blood or spleen. Neuraminidase augmented the binding of 125I-labeled antimacrophage globulins (AMG) and normal rabbit globulins to monocytes/macrophages.
UR - http://www.scopus.com/inward/record.url?scp=0015415412&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(72)90058-5
DO - 10.1016/0008-8749(72)90058-5
M3 - Article
C2 - 4116116
AN - SCOPUS:0015415412
SN - 0008-8749
VL - 5
SP - 325
EP - 337
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -