Distinguishing the effects of convective and diffusive O2 delivery on V̇o2 on-kinetics in skeletal muscle contracting at moderate intensity

Jessica Spires, L. Bruce Gladden, Bruno Grassi, Matthew L. Goodwin, Gerald M. Saidel, Nicola Lai

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

With current techniques, experimental measurements alone cannot characterize the effects of oxygen blood-tissue diffusion on muscle oxygen uptake (VO2) kinetics in contracting skeletal muscle. To complement experimental studies, a computational model is used to quantitatively distinguish the contributions of convective oxygen delivery, diffusion into cells, and oxygen utilization to VO2 kinetics. The model is validated using previously published experimental VO2 kinetics in response to slowed blood flow (Q) on-kinetics in canine muscle (τQ = 20 s, 46 s, and 64 s) [Goodwin ML, Hernández A, Lai N, Cabrera ME, Gladden LB. J Appl Physiol. 112:9-19, 2012]. Distinctive effects of permeability-surface area or diffusive conductance (PS) and Q on VO2 kinetics are investigated. Model simulations quantify the relationship between PS and Q, as well as the effects of diffusion associated with PS and Q dynamics on the mean response time of VO2. The model indicates that PS and Q are linearly related and that PS increases more with Q when convective delivery is limited by slower Q dynamics. Simulations predict that neither oxygen convective nor diffusive delivery are limiting VO2 kinetics in the isolated canine gastrocnemius preparation under normal spontaneous conditions during transitions from rest to moderate (submaximal) energy demand, although both operate close to the tipping point.

Original languageEnglish
Pages (from-to)R512-R521
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume305
Issue number5
DOIs
StatePublished - Sep 1 2013
Externally publishedYes

Keywords

  • Diffusion
  • Modeling
  • Permeability-surface area
  • Tipping point
  • Transport

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